Application of electrospun polycaprolactone fibers embedding lignin nanoparticle for peripheral nerve regeneration: In vitro and in vivo study
Application of electrospun polycaprolactone fibers embedding lignin nanoparticle for peripheral nerve regeneration: In vitro and in vivo study
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DOI：
10.1016/j.ijbiomac.2020.05.073
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发表时间：
2020-09-15
影响因子：
8.2
通讯作者：
Salehi, Hossein
中科院分区：
文献类型：
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作者：
Amini, Shahram;Saudi, Ahmad;Salehi, Hossein
木质素在外周神经应用中显示出有吸引力的特性。在这里，使用静电纺丝方法制造具有不同百分比的木质素纳米颗粒的排列的聚己内酯（PCL）纤维。研究了PCL/木质素纤维的形态、接触角、力学性能、体外降解性能和吸水性能。分别采用MTT法和扫描电镜观察PC 12和人脂肪干细胞（hADSCs）的细胞活力和粘附性。扫描电镜、免疫细胞化学和Real-Time PCR检测PC 12和hADSCs的神经分化和突起长度。为了进一步研究木质素对神经再生的影响，进行了体内研究。结果表明，所有的纳米复合纤维是光滑的，无珠。与PCL纤维相比，随着木质素含量的增加，水接触角减小，而体外降解、吸水率和杨氏模量增加。细胞活力，分化沿着与神经突长度的延长，促进了木质素含量的增加。随着木质素含量的增加，分化细胞的神经标志物表达上调。体内研究还表明，掺入15%木质素纳米颗粒的样品组显示出更好的再生等。因此，含有15%木质素纳米颗粒的PCL显示出巨大的应用于神经再生的潜力。(C)2020 Elsevier B. V.保留所有权利。
Lignin displays attractive properties in peripheral nerve applications. Here, aligned polycaprolactone (PCL) fibers with various percentages of lignin nanoparticles were fabricated using the electrospinning method. The mor-phologies, contact angles, mechanical properties, in vitro degradation, and water uptake of the PCL/lignin fibers were characterized. Cell viability and adhesion of PC12 and human adipose-derived stem cells (hADSCs) were studied employing MTT assay and SEM, respectively. SEM, immunocytochemistry, and Real-Time PCR were uti-lized to characterize neural differentiation and neurite length of PC12 and hADSCs. To further study on lignin ef-fect on nerve regeneration, in vivo studies were performed. The results indicated that all nanocomposite fibers were smooth and bead-free. With increasing the lignin content, the water contact angle decreased while in vitro degradation, water uptake, and Young's modulus increased compared to the PCL fibers. Cell viability, and differentiation along with neurite length extension were promoted by increasing lignin content. The neural markers expression for differentiated cells were upregulated by the increase of lignin percent. In vivo investiga-tion also demonstrates that sample groups incorporating 15% lignin nanoparticles showed better regeneration among others. Therefore, PCL with 15% of lignin nanoparticles shows great potential to be applied for nerve regeneration. (C) 2020 Elsevier B.V. All rights reserved.