New scheme of the biosynthesis of formononetin involving 2,7,4′-trihydroxyisofiavanone but not daidzein as the methyl acceptor
New scheme of the biosynthesis of formononetin involving 2,7,4′-trihydroxyisofiavanone but not daidzein as the methyl acceptor
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DOI：
10.1271/bbb.64.2276
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发表时间：
2000-10-01
影响因子：
1.6
通讯作者：
Ayabe, S
中科院分区：
文献类型：
--
作者：
Akashi, T;Sawada, Y;Ayabe, S
刺甘草无细胞提取物通过S-腺苷-L-蛋氨酸(SAM)中大豆苷元的7-O-甲基化生成芒柄花素，表达2-羟基异黄酮合酶的酵母微体与无细胞提取物混合后与甘草素和SAM孵育，得到芒柄花素，再与2，7，4‘-三羟基异黄酮和SAM孵育产生芒柄花素。我们提出了一种以2，7，4‘-三羟基异黄酮为甲基受体的芒柄花素生物合成新途径。
Glycyrrhiza echinata cell-free extract produced isoformononetin by the 7-O-transmethylation of daidzein from S-adenosyl-L-methionine (SAM), When the yeast microsome expressing 2-hydroxyisoflavanone synthase was mixed with the cell-free extract and incubated with liquiritigenin and SAM, formononetin emerged, Furthermore, the cell-free extract yielded formononetin on incubation with 2,7,4'-trihydroxyisoflavanone and SAM. We propose a novel pathway of formononetin biosynthesis involving 2,7,4'-trihydroxyisoflavanone as the methyl acceptor.