Responses of microRNAs 124a and 223 following spinal cord injury in mice
Responses of microRNAs 124a and 223 following spinal cord injury in mice
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DOI：
10.1038/sc.2009.89
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发表时间：
2010-03-01
期刊：
影响因子：
2.2
通讯作者：
Ochi, M.
中科院分区：
文献类型：
--
作者：
Nakanishi, K.;Nakasa, T.;Ochi, M.
研究设计：我们研究了小鼠脊髓损伤 (SCI) 后 microRNA (miRNA) 的表达。 目的：miRNA 的最新发现表明植物和动物发育过程中基因表达的新调控。 miRNA 是短非编码 RNA，通过与 mRNA 结合来抑制靶基因的翻译，并在健康和疾病的基因调控中发挥核心作用。本研究的目的是检测 SCI 后 miRNA 的表达。地点：广岛大学生物医学科学研究生院整形外科系。方法：我们使用定量 PCR 检测了 SCI 后 6 小时、12 小时、1 天、3 天和 7 天的小鼠模型中 miRNA (miR)-223 和 miR-124a 的表达。通过原位杂交证实miRNA表达。结果：定量PCR显示miR-223表达在SCI后6、12小时和3天出现两个峰。 SCI 后 1 天至 7 天，miR-124a 表达显着下降。原位杂交证明损伤部位周围存在 miR-223。然而，存在于正常脊髓中的 miR-124a 在损伤部位没有观察到。结论：我们的结果表明 SCI 小鼠模型中 miR-223 和 miR-124a 具有时间依赖性表达模式。本研究中，miRNA-223表达的时程可能与SCI后的炎症反应有关，而miR-124a表达下降的时程可能反映了细胞死亡。脊髓 (2010) 48, 192-196; doi：10.1038/sc.2009.89； 2009 年 7 月 21 日在线发布
Study design: We investigated microRNA (miRNA) expression after spinal cord injury (SCI) in mice.Objectives: The recent discovery of miRNAs suggests a novel regulatory control over gene expression during plant and animal development. MiRNAs are short noncoding RNAs that suppress the translation of target genes by binding to their mRNAs, and play a central role in gene regulation in health and disease. The purpose of this study was to examine miRNA expression after SCI.Setting: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University.Methods: We examined the expression of miRNA (miR)-223 and miR-124a in a mouse model at 6 h, 12 h, 1 day, 3 days and 7 days after SCI using quantitative PCR. The miRNA expression was confirmed by in situ hybridization.Results: Quantitative PCR revealed two peaks of miR-223 expression at 6 and 12 h and 3 days after SCI. MiR-124a expression decreased significantly from 1 day to 7 days after SCI. In situ hybridization demonstrated the presence of miR-223 around the injured site. However, miR-124a, which was present in the normal spinal cord, was not observed at the injured site.Conclusion: Our results indicate a time-dependent expression pattern of miR-223 and miR-124a in a mouse model of SCI. In this study, the time course of miRNA-223 expression may be related to inflammatory responses after SCI, and the time course of decreased miR-124a expression may reflect cell death. Spinal Cord (2010) 48, 192-196; doi: 10.1038/sc.2009.89; published online 21 July 2009