Stimulation of nitric oxide mechanotransduction in single osteoblasts using atomic force microscopy
Stimulation of nitric oxide mechanotransduction in single osteoblasts using atomic force microscopy
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DOI：
10.1002/jor.20515
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发表时间：
2008-04-01
影响因子：
2.8
通讯作者：
Jarvis, Suzanne P.
中科院分区：
文献类型：
--
作者：
McGarry, James G.;Maguire, Paula;Jarvis, Suzanne P.
从机械敏感骨细胞释放的一氧化氮（NO）在骨结构适应其机械使用中起着关键作用。尽管它在骨中很重要，但在细胞水平上参与NO机械转导的机制尚不清楚。利用原子力显微镜和荧光显微镜的结合，我们报道了在成骨细胞膜上施加定量的周期性压痕力诱导的单个成骨细胞的NO反应的刺激和实时监测。峰部队从17到50 nN刺激三个不同的缩进成骨细胞中没有反应:(1)快速和持续扩散的没有细胞核周围的地区,(2)没有从局部扩散池在成骨细胞,和(3)最初的增加和随后的细胞内没有下降。力压痕特征显示成骨细胞间弹性有相当大的变化。NO反应与施加更多刚性膜部位的力有关，表明细胞骨架参与了机械转导。(c) 2007年骨科研究学会。Wiley期刊公司出版。
Nitric oxide (NO) released from mechanosensitive bone cells plays a key role in the adaptation of bone structure to its mechanical usage. Despite its importance in bone, the mechanisms involved in NO mechanotransduction at the cellular level remain unknown. Using combined atomic force microscopy and fluorescence microscopy, we report both stimulation and real-time monitoring of NO responses in single osteoblasts induced by application of quantified periodic indenting forces to the osteoblast membrane. Peak forces ranging from 17 to 50 nN stimulated three distinct NO responses in the indented osteoblasts: (1) a rapid and sustained diffusion of NO from the perinuclear region, (2) diffusion of NO from localized pools throughout the osteoblast, and (3) an initial increase and subsequent drop in intracellular NO. Force-indentation characteristics showed considerable interosteoblast variation in elasticity. NO responses were associated with application of force to more rigid membrane sites, suggesting cytoskeletal involvement in mechanotransduction. (c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.