Imaging of ischemia and infarction in blood-perfused pig hearts using 87Rb MRI
Imaging of ischemia and infarction in blood-perfused pig hearts using 87Rb MRI
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DOI：
10.1002/mrm.10338
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发表时间：
2003-01-01
影响因子：
3.3
通讯作者：
Kuzio, B
中科院分区：
文献类型：
--
作者：
Kupriyanov, VV;Xiang, B;Kuzio, B
Rb-87-MRI用于测量Rb+摄取在血液灌注的猪心脏在完全闭塞（120和70分钟）的左前降支（LAD）和随后的再灌注（120和170分钟）。在120分钟闭塞期间，缺血性左心室（LV）前壁（0.35 +/- 0.14%/min和26 +/- 4.7%）的Rb+摄取率和最大Rb图像强度显著低于远端左心室后壁（2.43 +/- 0.33%/min和98 +/- 10%）。闭塞120和70分钟后再灌注导致形成损伤区域，在远端后壁观察到Rb-87图像强度的40 - 4和73 +/- 10%。通过氯化三苯基四氮唑染色组织学测定的梗死面积在120分钟和70分钟闭塞组中分别为总心室质量（LV + RV）的9.6 +/- 3.4和5.6 +/- 4.6%。通过MRI确定的大小分别为像素总数的13.1 +/- 2和2.8 +/- 4.3%。120分钟闭塞期间前壁的Rb+摄取略低于之前在晶体灌注心脏中观察到的结果。它的结论是，血液不干扰Rb-87 MRI检测缺血和梗死区的能力。
Rb-87-MRI was used to measure Rb+ uptake in blood-perfused pig hearts during complete occlusion (120 and 70 min) of the left anterior descending artery (LAD) and subsequent reperfusion (120 and 170 min). The Rb+ uptake rate and maximal Rb image intensity during 120-min occlusion were significantly lower in the ischemic anterior left ventricular (LV) wall (0.35 +/- 0.14%/min and 26 +/- 4.7%) relative to those in the remote posterior LV wall (2.43 +/- 0.33%/min and 98 +/- 10%). Reperfusion after 120 an 70 min of occlusion resulted in formation of damaged areas, which had 40 4 and 73 +/- 10% of the Rb-87 image intensity observed in the remote posterior wall. The infarct sizes determined histologically by triphenyltetrazolium chloride staining were 9.6 +/- 3.4 and 5.6 +/- 4.6% of the total ventricular mass (LV + RV) in the 120- and 70-min occlusion groups, respectively. The sizes determined by MRI were 13.1 +/- 2 and 2.8 +/- 4.3% of the total number of pixels, respectively. The Rb+ uptake in the anterior wall during 120-min occlusion was somewhat lower than that previously observed in crystalloid-perfused hearts. It is concluded that blood does not interfere with the ability of Rb-87 MRI to detect ischemic and infarct areas.