Association of black-pigmented bacteria with endodontic infections
Association of black-pigmented bacteria with endodontic infections
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DOI：
10.1016/s0099-2399(99)80268-4
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发表时间：
1999-06-01
影响因子：
4.2
通讯作者：
Xia, T
中科院分区：
文献类型：
--
作者：
Baumgartner, JC;Watkins, BJ;Xia, T
黑色素细菌（BPB）与牙髓感染有关，本研究的目的是进一步评估 BPB 的存在以及与牙髓感染相关的临床体征和症状。从 40 颗存在牙髓坏死和根尖周炎的完整牙齿的根管中采集了微生物样本。采用常规实验室方法对纯培养物中分离的BPB菌株进行鉴定，并采用聚合酶链式反应和16S r-RNA基因特异性引物区分Puevotella nigrescens和Puevotella intermedia。 22 个 (55%) 个样本 BPB 生长呈阳性，其中 22 个样本中的 11 个样本 (50%) 被鉴定为 P. nigrescens，22 个样本中的 8 个样本 (36%) 被鉴定为 P. intermedia，22 个样本中的 2 个样本 (9%) 被鉴定为牙龈卟啉单胞菌，22 个样本中的 1 个样本 (5%) 被鉴定为黑素普氏菌，22 个根中的 16 个样本BPB 生长呈阳性的根管与脓性引流相关 来自根管或相关窦道。统计分析并未显示 BPB 的存在与临床体征和症状之间存在显着关系。
Black-pigmented bacteria (BPB) have been associated with endodontic infections, The purpose of this study was to evaluate further the presence of BPB with the clinical signs and symptoms associated with endodontic infections. Microbial samples were collected from the root canals of 40 intact teeth with necrotic pulps and apical periodontitis. Conventional laboratory methods were used for identification of the strains of BPB isolated in pure culture, In addition, the polymerase chain reaction and specific primers for 16S r-RNA genes were used to differentiate Puevotella nigrescens from Puevotella intermedia. Twenty-two (55%) samples were positive for the growth of BPB, Of those, 11 of 22 (50%) were identified as P. nigrescens, 8 of 22 (36%) were P. intermedia, 2 of 22 (9%) were Porphyromonas gingivalis, and 1 of 22 (5%) was Prevotella melaninogenica, Sixteen of the 22 root canals positive for the growth of BPB were associated with purulent drainage either from the root canal or an associated sinus tract. Statistical analysis did not show a significant relationship for the presence of BPB with clinical signs and symptoms.