Association of Minimal Residual Disease by a Single-Tube 8-Color Flow Cytometric Analysis With Clinical Outcome in Adult B-Cell Acute Lymphoblastic Leukemia
Association of Minimal Residual Disease by a Single-Tube 8-Color Flow Cytometric Analysis With Clinical Outcome in Adult B-Cell Acute Lymphoblastic Leukemia
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单管 8 色流式细胞仪分析微小残留病与成人 B 细胞急性淋巴细胞白血病临床结果的关联
DOI：
10.5858/arpa.2022-0172-oa
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Qin Zheng
中科院分区：
文献类型：
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作者：
Hongyan Liao;Nenggang Jiang;Ying Yang;Xin Zhang;Jiao Chen;Hongli Lai;Qin Zheng
背景。通过分子和多参数流式细胞术（MFC）测量的微小/可测量残留病（MRD）已被证明可预测B细胞急性淋巴细胞白血病（B-ALL）患者的复发和生存。一个普遍适用的抗体面板在低成本，但不损害敏感性和能力的预后预测成人B-ALL仍然没有建立。客观。报告我们使用单管8色MFC面板测量MRD状态作为成人B-ALL患者预后指标的经验。设计。-。我们回顾性分析了成人B-ALL的特点，MRD状态和预后，基于一个10年期间486例患者的大型真实世界队列。结果。-。通过MFC和聚合酶链反应（PCR）检测BCR-ABL+患者的MRD评估显示，74.2%的病例结果一致。我们的MFC面板的MRD-状态可以清楚地预测诱导结束时和1个巩固疗程结束时的无复发生存期（RFS）和总生存期（OS）。与至少有1个MRD+结果和连续MRD+的患者相比，连续MRD−和至少有1个MRD−结果的患者的RFS和OS分别有利。结论。单管8色MFC板显示出低成本、良好的灵敏度和与聚合酶链反应-MRD的可比性，但在预测RFS和OS方面具有优异的性能，因此可以潜在地作为评价成人B-ALL患者治疗反应的常规指标。
Context.—. Minimal/measurable residual disease (MRD) measured by molecular and multiparametric flow cytometry (MFC) has been proven to be predictive of relapse and survival in patients with B-cell acute lymphoblastic leukemia (B-ALL). A universally applicable antibody panel at a low cost but without compromising sensitivity and power of prognosis prediction in adult B-ALL remains unestablished.. Objective.—. To report our experience of using a single-tube 8-color MFC panel to measure the MRD status as a prognostic indicator in adult B-ALL patients.. Design.—. We retrospectively analyzed the characteristics, MRD status, and prognosis of adult B-ALL based on a large real-world cohort of 486 patients during a 10-year period.. Results.—. MRD assessed by MFC and polymerase chain reaction (PCR) assays for BCR-ABL+ patients showed concordant results in 74.2% of cases. MRD− status by our MFC panel could clearly predict a favorable relapse-free survival (RFS) and overall survival (OS) both at the end of induction and at the end of 1 consolidation course. Patients with continuous MRD− and with at least 1 MRD− result showed a favorable RFS and OS compared with those with at least 1 MRD+ result and continuous MRD+, respectively.. Conclusions.—. The single-tube 8-color MFC panel demonstrated a low cost, decent sensitivity, and comparability with polymerase chain reaction–MRD but an excellent performance in predicting RFS and OS, and thus could potentially be taken as a routine indicator in the evaluation of the treatment response for adult patients with B-ALL.