The regulatory role of NZB T lymphocytes in the production of anti-DNA antibodies in vitro.
The regulatory role of NZB T lymphocytes in the production of anti-DNA antibodies in vitro.
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NZB T 淋巴细胞在体外抗 DNA 抗体产生中的调节作用。
DOI：
10.4049/jimmunol.137.6.1867
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发表时间：
1986
影响因子：
4.4
通讯作者：
C. Solonínka
中科院分区：
文献类型：
--
作者：
C. Laskin;G. Haddad;C. Solonínka
鼠狼疮的特征是产生大量自身抗体和免疫复合物肾小球肾炎。抗 DNA 抗体是这种疾病的标志，可能与肾小球肾炎的发病机制有关。抗 DNA 抗体产生调节的细胞机制可能被证明是导致这些小鼠狼疮综合征的根本异常。通过使用体外自发抗DNA抗体产生系统，我们确定这种产生是自身免疫NZB和MRL-lpr/lpr小鼠的特征，但不是非自身免疫对照品系的特征。对 NZB 小鼠中参与调节这种反应的细胞机制进行的额外检查显示：1）这种反应明显依赖于 T 细胞，2）NZB T 细胞对于最大限度地产生这种自身抗体至关重要，3）NZB T 细胞积极干扰正常的免疫调节机制，导致非自身免疫同基因 B 在体外自发产生抗 DNA 抗体 淋巴细胞。尽管这些体外抗 DNA 抗体生产的研究与其他人之前的工作不一致，但它们成功地重现了早期在体内进行的实验中获得的结果。
Murine lupus is characterized by the production of numerous autoantibodies and immune complex glomerulonephritis. Anti-DNA antibodies are the hallmark of this disorder and may be associated pathogenetically with the glomerulonephritis. The cellular mechanisms underlying the regulation of the production of anti-DNA antibodies may prove to be the fundamental abnormalities responsible for the lupus syndrome seen in these mice. By using a system of spontaneous anti-DNA antibody production in vitro, we have determined that such production is characteristic of autoimmune NZB and MRL-lpr/lpr mice but not of the nonautoimmune control strains. Additional examination of the cellular mechanisms involved in the regulation of this response in NZB mice revealed: 1) this response is markedly T cell dependent, 2) NZB T cells are essential for maximal production of this autoantibody, and 3) NZB T cells actively interfere with normal immune regulatory mechanisms that lead to the production of anti-DNA antibodies spontaneously in vitro by nonautoimmune syngeneic B lymphocytes. Although these studies of anti-DNA antibody production in vitro disagree with previous work by others they successfully reproduce the results obtained earlier in experiments performed in vivo.