USE OF STAPHYLOCOCCAL PROTEIN-A AS AN IMMUNOLOGICAL REAGENT
USE OF STAPHYLOCOCCAL PROTEIN-A AS AN IMMUNOLOGICAL REAGENT
复制标题
DOI：
10.1016/0022-1759(78)90259-4
复制
发表时间：
1978-01-01
影响因子：
2.2
通讯作者：
GODING, JW
中科院分区：
文献类型：
--
作者：
GODING, JW
本文简要综述了葡萄球菌蛋白A在免疫学中的主要应用。蛋白A与大多数金黄色葡萄球菌菌株的细胞壁共价结合，并以高亲和力结合免疫球蛋白[Ig]分子。主要的分子结合是IgG，尽管在许多情况下结合仅限于某些IgG亚类。一些IgM和IgA在某些物种中结合。这一特性使得用快速、简单和经济的方法纯化和分析Ig，以及用其他方法难以分离的亚类。在蛋白A亲和柱上分离是一种简单有效的从Fc片段中分离Ig F（ab）和F(ab ")2的方法。完整的葡萄球菌是一种有用的固相吸附剂，用于分离抗原-抗体复合物、膜抗原和受体，并在放射免疫测定中取代第二抗体。蛋白A已被证明对完整细胞表面的抗原和受体的研究以及抗体分泌细胞的检测很有用。蛋白A的使用现在是免疫学中许多制备和分析目的的首选方法。
This brief review summarizes the major uses of staphylococcal protein A in immunology. Protein A is covalently linked to the cell wall of most strains of Staphylococcus aureus, and binds immunoglobulin [Ig] molecules with high affinity. The principal molecule bound is IgG, although in many cases binding is restricted to certain IgG subclasses. Some IgM and IgA binds in certain species. This property allows rapid, simple and economical methods for the purification and analysis of Ig, and the fractionation of subclasses which are difficult to separate by other means. Fractionation on protein A affinity columns is a simple and efficient way of separating Ig F(ab) and F(ab'')2 from Fc fragments. Intact staphylococci are useful as a solid phase adsorbent for isolating antigen-antibody complexes, membrane antigens and receptors, and to replace 2nd antibody in radioimmunoassay. Protein A has proven useful for the study of antigens and receptors on the surface of intact cells and for the detection of antibody-secreting cells. The use of protein A is now the method of choice for many preparative and analytical purposes in immunology.