Stimulation of Skin Menthol Receptors Stimulates Detrusor Activity in Conscious Rats
Stimulation of Skin Menthol Receptors Stimulates Detrusor Activity in Conscious Rats
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刺激皮肤薄荷醇受体可刺激清醒大鼠的逼尿肌活动
DOI：
10.1002/nau.20774
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发表时间：
2010
影响因子：
2
通讯作者：
今村哲也
中科院分区：
文献类型：
--
作者：
陳忠;石塚修;今村哲也
瞬时受体电位Melastatin（TRPM）8通道被薄荷醇和低温激活，被认为是一种冷受体，参与了诊断性冰水试验中膀胱反应的介导。我们研究了TRPM 8在大鼠皮肤中的表达，并研究了如果刺激皮肤薄荷醇受体的薄荷醇可以诱导逼尿肌活动在清醒rat.MethodsImmunofluorescence染色TRPM 8进行从腿部和背部的大鼠皮肤样品。在清醒动物中进行连续膀胱测压。排尿间隔（VI），排尿量（MV）和膀胱容量（BC）进行了评价之前和之后，喷雾薄荷醇溶液的皮肤的腿和背部的rats. ResultsImmunohistochemistry分析显示，TRPM 8在调查组织中表达。TRPM 8阳性受体的表达在腿部和背部皮肤之间没有显著差异。生理盐水未引起膀胱测压参数的显著变化。结果表明，大鼠皮肤中TRPM 8的表达量明显低于对照组（P < 0. 05），而在小鼠皮肤中则明显低于对照组（P < 0. 05）。将薄荷醇溶液喷洒到皮肤上诱导逼尿肌活动，我们认为这种作用是通过刺激TRPM 8受体介导的。Neurorol。Urodynam。29：506-511，2010.© 2009 Wiley利斯公司
AimsThe Transient Receptor Potential Melastatin (TRPM)8 channel, activated by menthol and cool temperatures, has been proposed to be a cool receptor involved in mediation of the bladder response in the diagnostic ice water test. We examined the expression of TRPM8 in the rat skin and investigated if stimulation of skin menthol receptors by menthol can induce detrusor activity in conscious rats.MethodsImmunofluorescence staining for TRPM8 was performed in samples of the skin from the leg and back of the rats. Continuous cystometry was performed in conscious animals. Voiding interval (VI), micturition volume (MV), and bladder capacity (BC) were evaluated before and after spraying menthol solution to the skin of the leg and back of the rats.ResultsImmunohistochemical analysis revealed that TRPM8 was expressed in the investigated tissues. The expression of TRPM8‐positive receptors was not significantly different between the leg and back skin. Saline caused no significant change in cystometric parameters. After spraying menthol solution (50% and 99% to the skin of the leg, and 99% to the back skin), VI, MV, and BC decreased significantly.ConclusionsTRPM8 is expressed in rat skin. Spraying menthol solution onto the skin‐induced detrusor activity, an effect we suggest is mediated by stimulation of TRPM8 receptors. Neurourol. Urodynam. 29:506–511, 2010. © 2009 Wiley‐Liss, Inc.