α7-nicotinic receptor expression and the anatomical organization of hippocampal interneurons
α7-nicotinic receptor expression and the anatomical organization of hippocampal interneurons
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DOI：
10.1016/s0006-8993(01)03115-8
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发表时间：
2001-12-20
期刊：
影响因子：
2.9
通讯作者：
Freedman, R
中科院分区：
文献类型：
--
作者：
Adams, CE;Stitzel, JA;Freedman, R
C3 H和DBA/2小鼠海马抑制功能不同，通过对重复听觉刺激的锥体神经元反应的抑制门控来测量。这种功能差异似乎与α 7烟碱胆碱能受体表达的差异有关，α 7烟碱胆碱能受体通常由中间神经元表达。本研究探讨了两个品系小鼠海马不同区域和层次中α 7受体亚单位表达的遗传变异与GABA能中间神经元分布之间的关系。发现两种小鼠品系的海马中间神经元亚群与[I-125] α-银环蛇毒素结合。然而，C3 H和DBA/2小鼠之间[I-125] α-银环蛇毒素阳性海马中间神经元的分布显着不同。在CA 1区。并且在区域CA 3中程度较低。DBA/2小鼠腔隙分子层中[I-125] α-银环蛇毒素阳性神经元数量增加，而定向层中神经元数量减少。在两个品系的CA 1区观察到GABA神经元分布的类似差异。将C3 H/DBA/2 F1动物与C3 H亲本品系回交6代，选择α 7受体基因的DBA/2或C3 H等位基因变体。[I-125] α-银环蛇毒素标记的分布与保留α 7基因的DBA/2等位基因的动物中的DBA/2亲本表型非常相似。这些数据表明，α 7受体基因位点可能会影响解剖组织的至少一个子集的海马中间神经元的一个尚未确定的机制。中间神经元解剖结构的这种差异也可能导致两种菌株之间抑制性感觉门控的功能差异。(C)2001 Elsevier Science B. V.保留所有权利。
C3H and DBA/2 mice differ in their hippocampal inhibitory function, as measured by the inhibitory gating of pyramidal neuron response to repeated auditory stimulation. This functional difference appears to be related to differences in expression of the alpha7 nicotinic cholinergic receptor, which may be generally expressed by interneurons. This study examines the relationship between genetic variation in alpha7 receptor subunit expression and GABAergic interneuron distribution in various regions and layers of the hippocampus in the two Mouse strains. Subpopulations of hippocampal interneurons in both mouse strains were found to bind [I-125]alpha-bungarotoxin. However, the distribution of the [I-125]alpha-bungarotoxin-positive hippocampal interneurons was significantly different between C3H and DBA/2 mice. In region CA1. and to a lesser extent in region CA3. DBA/2 mice had increased numbers of [I-125]alpha-bungarotoxin-positive neurons in stratum lacunosum-moleculare and decreased numbers in stratum oriens. Similar differences in GABAergie neuron distribution were observed in region CA1 in the two strains. C3H/DBA/2 F1 animals were backcrossed to the C3H parental strain for six generations, with selection for either the DBA/2 or C3H allelic variant of the alpha7 receptor gene. The distribution of [I-125]alpha-bungarotoxin labeling closely resembled the DBA/2 parental phenotype in animals retaining the DBA/2 allele of the alpha7 gene. These data suggest that the alpha7 receptor gene locus may influence the anatomical organization of at least a subset of hippocampal interneurons by an as yet unidentified mechanism. This difference in interneuron anatomy may also contribute to functional differences in inhibitory sensory gating between the two strains. (C) 2001 Elsevier Science B.V. All rights reserved.