Effect of Exogenous Cadaverine Phosphate on Plant Growth, Photosynthesis, and Gene Expression of Lettuce Seedlings
Effect of Exogenous Cadaverine Phosphate on Plant Growth, Photosynthesis, and Gene Expression of Lettuce Seedlings
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DOI：
10.1007/s42729-023-01563-2
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发表时间：
2023-12-05
影响因子：
3.9
通讯作者：
Gao，Nan
中科院分区：
文献类型：
--
作者：
Zhou，Chaowei;Zheng，Yilin;Gao，Nan
过度使用处理化学品和化肥对农业生产力构成严重威胁。开发新型生物基叶面剂是促进植物生长和减轻盐胁迫毒性的最佳环境和经济可行的选择。尸胺磷酸（CP）在莴苣（Lactuca sativaL）产量、光合作用和转录表达中的潜在作用。研究了温室内盐碱地和非盐碱地上生长的拉莫沙（var. ramosa, short .）幼苗。盆栽试验中，在正常生长条件下，施用1 g L−1 cp对生菜生长有促进作用，但在盐胁迫条件下效果不明显。喷施CP 24 h后，与蒸馏水对照相比，在正常和生理盐水条件下，净光合速率分别显著提高了75%和46.5%。RNA-seq分析显示，6 h时芽和根的差异表达基因（DEGs）分别为486和104个，24 h时芽和根的差异表达基因（DEGs）分别为31和117个。京都基因百科和基因组富集分析表明，CP可调控倍半萜类和三萜类生物合成、苯丙类生物合成、氰氨基酸代谢、植物激素信号转导、丝裂原活化蛋白激酶信号通路和光合作用相关的基因表达。总的来说，CP提高了生菜的光合能力，诱导了缓解胁迫的基因，从而达到了促进生菜生长的效果。该研究揭示了有机磷促进植物生长的分子机制，为提高植物生物肥料施用的稳定性提供了理论依据。
Excessive use of treatment chemicals and fertilizers poses a severe threat to agricultural productivity. The development of novel biobased foliar agents is the best environmentally and economically viable option to promote plant growth and alleviate salt stress toxicity. The potential role of cadaverine phosphate (CP) in the productivity, photosynthesis, and transcriptional expression in lettuce (Lactuca sativaL. var. ramosa Hort.) seedlings grown in saline and non-saline soils in the greenhouse was investigated. Under normal growth conditions, the application of 1 g L−1CP promoted lettuce growth in pot experiments, but this effect was not evident under salt-stress conditions. After 24 h of spraying with CP, the net photosynthetic rate increased significantly by 75 and 46.5% under normal and saline conditions, respectively, compared with the distilled water control. RNA-seq analysis revealed 486 and 104 differentially expressed genes (DEGs) in the shoots and roots at 6 h and 31 and 117 DEGs at 24 h, respectively. Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that CP could regulate genetic expression in association with sesquiterpenoid and triterpenoid biosynthesis, phenylpropanoid biosynthesis, cyanoamino acid metabolism, plant hormone signal transduction, mitogen-activated protein kinase signaling pathway, and photosynthesis. Overall, CP enhanced the photosynthetic capacity, induced stress alleviation genes, and consequently achieved a growth-promoting effect on lettuce. This study provides insights into the molecular mechanisms underlying plant growth promotion by CP, which may be useful for enhancing the stability of biofertilizer applications in plants.