Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.)
Molecular detection of QTLs for agronomic and quality traits in a doubled haploid population derived from two Canadian wheats (Triticum aestivum L.)
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DOI：
10.1007/s00122-006-0346-7
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发表时间：
2006-08-01
影响因子：
5.4
通讯作者：
Brown, P. D.
中科院分区：
文献类型：
--
作者：
Huang, X. Q.;Cloutier, S.;Brown, P. D.
培育终端品质优良的高产小麦品种一直是小麦育种工作者关心的问题。为了对产量、株高、成熟度、倒伏、容重和千粒重等产量相关性状以及籽粒和面粉蛋白质含量、面筋强度等品质性状进行遗传分析，以加拿大小麦品种“AC Karma”与育种系87E03-S2B1杂交，建立了185个F-1代加倍单倍体群体。构建了由160个微卫星座位、Glu-A1、Glu-B1和Glu-D1 3个高分子量谷蛋白亚基座位和4个STS-PCR标记组成的167个标记座位的遗传图谱。所调查的性状数据是从加拿大马尼托巴省的三到四个环境中收集的。利用复合区间作图法进行QTL分析。共检测到50个QTL，其中农艺性状24个，品质相关性状26个。相关性状的多个QTL被定位在同一基因组区域，形成QTL簇。在1D和4D染色体上发现了最大的QTL簇，由多达9个QTL组成。Glu-1座位上的HMW麦谷蛋白亚基对面包品质的影响最大，但其他基因组区域也对面包品质有遗传影响。将本研究检测到的QTL与其他小麦QTL分析进行了比较。
Development of high-yielding wheat varieties with good end-use quality has always been a major concern for wheat breeders. To genetically dissect quantitative trait loci (QTLs) for yield-related traits such as grain yield, plant height, maturity, lodging, test weight and thousand-grain weight, and for quality traits such as grain and flour protein content, gluten strength as evaluated by mixograph and SDS sedimentation volume, an F-1-derived doubled haploid (DH) population of 185 individuals was developed from a cross between a Canadian wheat variety "AC Karma" and a breeding line 87E03-S2B1. A genetic map was constructed based on 167 marker loci, consisting of 160 microsatellite loci, three HMW glutenin subunit loci: Glu-A1, Glu-B1 and Glu-D1, and four STS-PCR markers. Data for investigated traits were collected from three to four environments in Manitoba, Canada. QTL analyses were performed using composite interval mapping. A total of 50 QTLs were detected, 24 for agronomic traits and 26 for quality-related traits. Many QTLs for correlated traits were mapped in the same genomic regions forming QTL clusters. The largest QTL clusters, consisting of up to nine QTLs, were found on chromosomes 1D and 4D. HMW glutenin subunits at Glu-1 loci had the largest effect on breadmaking quality; however, other genomic regions also contributed genetically to breadmaking quality. QTLs detected in the present study are compared with other QTL analyses in wheat.