Microfocus computed tomography analysis of early changes in bone microstructure in rats with chronic renal failure
Microfocus computed tomography analysis of early changes in bone microstructure in rats with chronic renal failure
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DOI：
10.1159/000074842
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发表时间：
2003-01-01
影响因子：
--
通讯作者：
Fukagawa, K
中科院分区：
文献类型：
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作者：
Kazama, JJ;Iwasaki, Y;Fukagawa, K
背景/目的：慢性肾功能衰竭(CRF)早期骨组织的微结构改变知之甚少。在这项研究中，我们使用了一种高分辨率的X射线断层扫描系统，即微焦点计算机断层扫描(MXCT)，对早期CRF大鼠骨组织进行了分析，该系统能够检测骨组织的微观结构变化。方法：采用7/8肾动脉分支结扎法建立CRF大鼠模型，术后24周处死动物。采用MXCT和单能X线骨密度仪(SXA)对提取的骨标本进行骨密度测定。对MXCT图像进行节点-支柱形态计量学分析。结果：生化分析证实7/8肾动脉结扎大鼠可诱发CRF。MXCT显示尿毒症大鼠的骨小梁显著减少，而SXA无法检测到这种差异。结节支撑法显示尿毒症大鼠骨小梁连通性降低，骨小梁厚度变化不大。结论：MXCT能在肾功能衰竭早期发现骨小梁的明显改变。观察到的结构变化与长期透析患者的不同。MXCT是无创评价尿毒症患者早期骨改变的一种实用而有前景的设备。版权所有(C)2003 S.Karger AG，巴塞尔。
Background/Aims: Little is known about the microstructural changes in bone tissue in the early phase of chronic renal failure (CRF). In this study, we analyzed the bone tissue in a rat model of early CRF using a high-resolution X-ray tomographic system, i.e. microfocus computed tomography (MXCT), which is capable of detecting microscopic structural changes in bone tissue. Methods: CRF rats were created by 7/8 nephrectomy by ligation of the renal artery branches, and were sacrificed 24 weeks after surgery. MXCT and bone mineral densitometry by single-energy X-ray absorptiometry (SXA) were performed using extracted bone samples. Images obtained by MXCT were further analyzed by node-strut morphometry. Results: Biochemical analysis confirmed that CRF was induced in 7/8-renalartery-ligated rats. MXCT demonstrated a significant decrease in trabecular bone in uremic rats, whereas SXA was unable to detect the difference. The node-strut method revealed decreased trabecular connectivity, with little change in the thickness of the trabecular bone in uremic rats. Conclusion: MXCT was able to detect significant changes in trabecular bone at an early stage of renal failure. The observed structural changes differed from those found in long-term dialysis patients. MXCT is a practical and promising device used for the non-invasive evaluation of early bone changes in uremic patients. Copyright (C) 2003 S. Karger AG, Basel.