Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition
Macroscopic 'degeneration' of equine superficial digital flexor tendon is accompanied by a change in extracellular matrix composition
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DOI：
10.1111/j.2042-3306.1998.tb04530.x
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发表时间：
1998-11-01
影响因子：
2.2
通讯作者：
Goodship, AE
中科院分区：
文献类型：
--
作者：
Birch, HL;Bailey, AJ;Goodship, AE
在需要快速奔跑和跳跃的马中，浅指屈肌腱的损伤是常见的，该肌腱的部分断裂通常发生在掌骨中部区域的中央核心，并且可能在局部退行性变化之前发生。对明显正常的马屈肌腱进行尸检，发现中央核心出现异常的宏观外观，其特征是红色变色。我们以前已经证明胶原纤维也有物理损伤。在本研究中，我们测试了这一假设，即异常外观伴随着肌腱细胞外基质组成的变化。细胞外基质的生化分析表明，总硫酸化糖胺聚糖含量的增加，III型胶原蛋白的比例增加和胶原蛋白相关的荧光减少在中央核心的“退化”肌腱相对于组织从周边地区相同的肌腱。干物质含量和总胶原含量没有显着差异肌腱区或正常和“退化”肌腱。这些变化表明肌腱中央核心的细胞代谢和基质周转发生了变化，并可能导致肌腱这一部分的机械性能下降，从而诱发肌腱特征性部分断裂。
Injuries to the superficial digital flexor tendon are common in horses required to gallop and jump at speed, Partial rupture of this tendon usually occurs in the central core of the mid-metacarpal region and may be preceded by localised degenerative changes. Post mortem examination of apparently normal equine flexor tendons has revealed an abnormal macroscopic appearance in the central core, characterised by a reddish discolouration, We have previously shown that there is also physical damage to the collagen fibres, In the present study we tested the hypothesis that the abnormal appearance is accompanied by changes in the composition of the extracellular matrix of the tendon. Biochemical analysis of the extracellular matrix demonstrated an increase in total sulphated glycosaminoglycan content, increase in the proportion of type III collagen and decrease in collagen linked fluorescence in the central core of 'degenerated' tendons relative to tissue from the peripheral region of the same tendon. Dry matter content and total collagen content were not significantly different between tendon zones or normal and 'degenerated' tendons. These changes suggest a change in cell metabolism and matrix turnover in the central core of the tendon and are likely to contribute to a decrease in mechanical properties in this part of the tendon, predisposing to the characteristic partial rupture of the tendon.