Metabolism of dietary procyanidins in rats
Metabolism of dietary procyanidins in rats
复制标题
DOI：
10.1016/s0891-5849(03)00394-0
复制
发表时间：
2003-10-15
影响因子：
7.4
通讯作者：
Scalbert, A
中科院分区：
文献类型：
--
作者：
Gonthier, MP;Donovan, JL;Scalbert, A
原花青素是由基本的黄烷-3-醇(EPI)儿茶素单元组成的主要膳食多酚。它们具有抗氧化特性，可能对人类健康有益，但人们对它们的代谢命运知之甚少。我们比较了原花青素二聚体B-3、三聚体C-2和从柳絮中分离出来的聚合物与儿茶素单体在大鼠体内的代谢。这些化合物在大鼠饲料中(0.1%，w/w)连续给药5d，并在24小时尿液中估计其代谢物。在喂食原花青素的大鼠中，既没有检测到母体化合物，也没有检测到儿茶素衍生物，而喂饲儿茶素单体的动物排泄儿茶素及其3‘-O-甲基化形式(25.7+/-0.6%)。另一方面，检测到16种微生物来源的代谢物，分别为苯丙酸、苯丙酸、苯乙酸衍生物和苯甲酸衍生物。总产率从儿茶素单体(10.6+/-1.1%)显著下降到原花青素二聚体(6.5+/-0.2%)、三聚体(0.7+/-0.1%)和聚合物(0.5+/-0.1%)。因此，与儿茶素相比，原花青素的聚合程度对它们在体内的命运有很大影响，其特征是通过肠道屏障的吸收较差，肠道微生物群的代谢有限。为了解释原花青素对健康的影响，必须考虑这一点。它们的微生物代谢物的贡献也应该进一步调查。(C)2003年爱思唯尔公司。
Procyanidins are major dietary polyphenols made of elementary flavan-3-ol (epi)catechin units. They have antioxidant properties and may contribute to health benefits in humans, but little is known about their metabolic fate. We compared here the metabolism of procyanidin dimer B-3, trimer C-2, and polymer isolated from willow tree catkins to that of catechin monomer in rats. These compounds were administered in the rat diet (0.1%, w/w) for 5 d and their metabolites estimated in 24 h urine. In rats fed procyanidins, neither parent compound nor catechin derivatives could be detected in contrast to animals fed catechin monomer, which excreted large amounts of catechin and its 3'-O-methylated form (25.7 +/- 0.6%). On the other hand, 16 metabolites of microbial origin were detected and identified as phenylvaleric, phenylpropionic, phenylacetic, and benzoic acid derivatives. Their total yields significantly decreased from the catechin monomer (10.6 +/- 1.1%) to the procyanidin dimer (6.5 +/- 0.2%), trimer (0.7 +/- 0.1%), and polymer (0.5 +/- 0.1%). Therefore, the degree of procyanidin polymerization has a major impact on their fate in the body characterized by a poor absorption through the gut barrier and a limited metabolism by the intestinal microflora as compared to catechin. This will have to be considered to explain the health effects of procyanidins. The contribution of their microbial metabolites should also be further investigated. (C) 2003 Elsevier Inc.