Development of an H215O steady-state method combining a bolus and slow increasing injection with a multiprogramming syringe pump
Development of an H215O steady-state method combining a bolus and slow increasing injection with a multiprogramming syringe pump
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DOI：
10.1038/jcbfm.2010.122
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发表时间：
2011-02-01
影响因子：
6.3
通讯作者：
Okazawa, Hidehiko
中科院分区：
文献类型：
--
作者：
Kobayashi, Masato;Kiyono, Yasushi;Okazawa, Hidehiko
建立了一种对小动物压力小、采血点少的O-15标记水（（H_2O）-O-15）稳态法定量测定脑血流量（CBF）。在使用剂量计进行模拟以实现血液中稳定的（H2O）-O-15放射性后，使用编程为缓慢增加注射体积的多程序注射泵，对10只大鼠进行了注射方法研究。在6分钟正电子发射断层扫描（PET）扫描期间，每分钟采集一次动脉血。PET扫描后，将N-异丙基-p-[I-125]-碘苯丙胺（I-125-IMP）注射到同一大鼠体内，使用基于微球模型的放射自显影法测量CBF。将感兴趣区域置于（H2O）-O-15-PET和I-125-IMP-放射自显影图像的全脑上，并比较两种方法计算的CBF值。剂量计中的放射性达到平衡，与开始（H2O）-O-15注射后1分钟相似。在大鼠研究中，血液和脑中的放射性在给药后2分钟迅速达到平衡。（H2O）-O-15 PET的CBF值（49.2 ± 5.4mL/100 g/min）与125 I-IMP放射自显影的CBF值（49.1 ± 5.2mL/100 g/min）相关性良好（y = 1.01x-0.37，r（2）= 0.97）。连续增加注射量的（H2O）-O-15稳态方法可用于小动物研究中的CBF测量，特别是当需要在同一动物中进行多次扫描时。Journal of Cerebral Blood Flow & Metabolism（2011）31，527-534; doi：10.1038/jcbfm.2010.122; 2010年7月28日在线发表
An O-15-labeled water ((H2O)-O-15) steady-state method for quantitative measurement of cerebral blood flow (CBF), which is less stressful to small animals with a few point blood sampling, was developed. After a simulation using a dose meter to achieve stable (H2O)-O-15 radioactivity in the blood with a multiprogramming syringe pump programmed for slowly increasing injection volume, 10 rats were studied with the injection method. Arterial blood was sampled every minute during 6-minute positron emission tomography (PET) scans. After the PET scan, N-isopropyl-p-[I-125]-iodoamphetamine (I-125-IMP) was injected into the same rat to measure CBF using the autoradiography method based on a microsphere model. Regions of interest were placed on the whole brain in (H2O)-O-15-PET and I-125-IMP-autoradiography images, and CBF values calculated from both methods were compared. Radioactivity in the dose meter achieved equilibrium similar to 1 minute after starting the (H2O)-O-15 injection. In rat studies, radioactivity in the blood and brain rapidly achieved equilibrium at 2 minutes after administration. The correlation of CBF values of (H2O)-O-15 PET (49.2 +/- 5.4mL per 100 g per minute) and those of 125I-IMP autoradiography (49.1 +/- 5.2mL per 100 g per minute) was excellent (y = 1.01x-0.37, r(2) = 0.97). The (H2O)-O-15 steady-state method with a continuously increasing injection is useful for CBF measurement in small animal studies, especially when multiple scans are required in the same animal. Journal of Cerebral Blood Flow & Metabolism (2011) 31, 527-534; doi: 10.1038/jcbfm.2010.122; published online 28 July 2010