EFFECT OF LOW-LEVEL OF DIETARY AFLATOXINS ON BALADI RABBITS
EFFECT OF LOW-LEVEL OF DIETARY AFLATOXINS ON BALADI RABBITS
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DOI：
10.1080/17450399009421084
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发表时间：
1990-05-01
期刊：
影响因子：
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通讯作者：
GAMIL, T
中科院分区：
文献类型：
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作者：
ABDELHAMID, AM;ELSHAWAF, I;GAMIL, T
在提供给Baladi兔的糊状物中以低浓度（每种黄曲霉毒素（AN）100 ppb）给予黄曲霉毒素B1、B2、G1和G2。另一组兔子除了喂食0.25%的木炭（CC）外，还喂食相同的污染醪。将这两组动物与饲喂不含AN的醪液的对照动物进行比较。饲料中添加AN可降低饲料和水的消耗量、体重和存活率。木炭组的饲料消耗量、饮水消耗量和生长速度比AN组有所提高。 但CC组对有机物消化率的影响大于AN组。肝、肾、心脏和肾上腺的相对重量在AN和CC组显著高于对照组。AN组血红蛋白含量、红细胞压积百分比、血沉均低于对照组。AN组血清钙、无机磷、胆固醇、磷脂及谷氨酰胺转氨酶均升高，而血清氮及谷氨酰胺转氨酶降低。木炭减轻了N、GPT和磷脂的AN效应。化学分析显示，从AN动物的肝脏和肌肉的水含量的水，灰分和二氧化硅含量的升高。另一方面，肝脏中的脂肪含量、GOT和维生素A以及肌肉灰分减少。在日粮中添加CC降低了AN对肝脏脂肪、灰分和二氧化硅的影响，但导致肝脏和肌肉的含水量和肝脏GPT活性上升。木炭还导致肝脏维生素A含量急剧下降。黄曲霉毒素治疗（在AN和CC组）减少骨灰，二氧化硅和镁以及骨体积。木炭给药增加骨钙含量。黄曲霉毒素喂养（AN组）导致AN在肌肉、血清、肝脏、心脏和肾脏中的高残留百分比，其关系分别为51：24：3：2：1。只有1.42%的AN通过粪便排出。木炭的使用有很好的效果，因为它阻止了AN在器官中的积累。黄曲霉毒素污染的饮食（在AN和CC组）导致瘫痪，脂肪沉积障碍，变色和出血的一些器官。扫描电子显微镜检查显示，由于AN或AN+CC对小肠的表面结构没有不良影响。病理学检查显示主要受累器官分别为肝、心和脾。肝圆细胞浸润、小叶板不规则、局灶性坏死、门静脉周围纤维化。心内膜下纤维化和成纤维细胞增生。脾脏充血。AN组和CC组均出现上述病理改变。
Aflatoxins B1, B2, G1 and G2 were administered in a low concentration (100 ppb of each aflatoxin (AN)) in a mash offered to Baladi rabbits. An other group of rabbits were fed on the same contaminated mash in addition to 0.25% charcoal (CC). The two groups were compared to control animals fed on AN-free mash. Inclusion of AN in the diet decreased feed and water consumption, body weight and survival rate. Charcoal improved somewhat feed and water consumption and growth rate than AN-group. However, CC-group affected digestibility of organic matter more than AN-group. Relative weights of liver, kidneys, heart and adrenal glands were significantly higher in AN and CC groups than the control group. Blood haemoglobin content, packed cell volume percentage and sedimentation rate were lower in AN group. Although there were an increase in each of serum, calcium, inorganic phosphorus, cholesterol, phospholipids and glutamic-pyruvic transaminase in AN group, yet the serum nitrogen and glutamic-oxalacetic transaminase were reduced. Charcoal had alleviated AN-effects concerning N, GPT and phospholipids. Chemical analysis revealed elevation of water, ash and silica contents of liver and water content of muscles from AN-animals. On the other hand, fat content, GOT and vitamin A in the liver as well as muscles ash were reduced. Addition of CC to the diet reduced AN-effects on liver fat, ash and silica but resulted in a rise of the water content of liver and muscles and liver GPT activity. Charcoal also resulted in a sharp decrease in vitamin A content of the liver. Aflatoxin treatments (in AN and CC groups) reduced bone ash, silica and magnesium as well as bone volume. Charcoal administration increased Ca-content of bones. Aflatoxin feeding (in AN group) resulted in a high residual percentage of AN in muscles, serum, liver, heart and kidneys with relationships of 51: 24: 3: 2: 1, respectively. Only 1.42% of the fed AN was excreted in the faeces. Charcoal usage had a good effect as it prevented AN to accumulate in the organs. Aflatoxin contaminated diets (in AN and CC groups) resulted in paralysis, disorder of fat deposition, discolouration and haemorrhages of some organs. Scanning electron microscopic examination revealed no ill effect on the surface structure of the small intestine due to either AN or AN+CC. Pathological examination showed that the main affected organs were liver, heart and spleen, respectively. The changes include hepatic round cell infiltration, irregularities of lobular plats, focal necrosis and periportal fibrosis. Subendocardial fibrosis and fibroblastic proliferation were the cardiac changes. The spleen was congested. All of the pathological changes were found in both groups fed on AN alone or with CC.