Antioxidant dietary supplementation in mice exposed to proton radiation attenuates expression of programmed cell death-associated genes.
Antioxidant dietary supplementation in mice exposed to proton radiation attenuates expression of programmed cell death-associated genes.
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DOI：
10.1667/rr2330.1
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发表时间：
2011-05
影响因子：
3.4
通讯作者：
Kennedy AR
中科院分区：
文献类型：
--
作者：
Sanzari JK;Wambi C;Lewis-Wambi JS;Kennedy AR
膳食抗氧化剂在电离辐射暴露后具有辐射防护作用，可限制造血细胞耗竭。我们试图确定接受中等剂量全身质子辐射的动物中质子诱导造血细胞死亡的机制。此外，动物维持补充或不补充膳食抗氧化剂的饮食。在饮食抗氧化剂存在的情况下，与未接受抗氧化剂制剂的受辐射小鼠的表达谱相比，细胞凋亡相关基因的总骨髓mRNA和蛋白质表达降低。这些数据证实了高能质子诱导的经典细胞凋亡标志物（包括 BAX、caspase-3 和 PARP-1）的基因表达。除了抗凋亡标记物 Bcl2 和 Bcl-xL 的蛋白表达增加之外，补充抗氧化剂还导致这些基因的表达减少。总之，口服补充抗氧化剂似乎是防止造血细胞死亡的辐射防护的有效方法。
Dietary antioxidants have radioprotective effects after ionizing radiation exposure that limit hematopoietic cell depletion. We sought to determine the mechanism of proton-induced hematopoietic cell death in animals receiving a moderate dose of whole-body proton radiation. In addition, animals were maintained on diets supplemented with or without dietary antioxidants. In the presence of the dietary antioxidants, total bone marrow mRNA and protein expression of apoptosis-related genes were decreased compared to the expression profiles in the irradiated mice not receiving the antioxidant formulation. These data confirm high-energy proton-induced gene expression of classical apoptosis markers including BAX, caspase-3 and PARP-1. Antioxidant supplementation resulted in decreased expression of these genes in addition to increased protein expression of the anti-apoptosis markers Bcl2 and Bcl-xL. In conclusion, oral supplementation with antioxidants appears to be an effective approach for radioprotection against hematopoietic cell death.