Study of hemoglobin response to mid-ultraviolet (UVB) radiation using micro-Raman spectroscopy
Study of hemoglobin response to mid-ultraviolet (UVB) radiation using micro-Raman spectroscopy
复制标题
使用微拉曼光谱研究血红蛋白对中紫外线 (UVB) 辐射的响应
DOI：
10.1007/s00340-017-6800-1
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发表时间：
2017-09-01
影响因子：
2.1
通讯作者：
Zhuang, Z. F.
中科院分区：
文献类型：
--
作者：
Huang, Y. Y.;Li, N.;Zhuang, Z. F.
利用共焦显微拉曼光谱监测中紫外线(UVB)辐射对血红蛋白的损伤。我们获得了红细胞的拉曼光谱，这表明UVB辐射后发生了过氧化反应。此外，分离的血红蛋白的表面增强拉曼散射(SERS)光谱表明，随着UVB照射时间的延长，作为血红蛋白聚集标志的1375和1399 cm(-1)谱带的强度明显增加。这一增加表明，血红素聚集发生在红细胞的过氧化过程中。分离出的血红蛋白的UV-Vis光谱表明，经UVB照射30分钟后，代表聚集的HEM中激子相互作用的Soret带发生了红移(约12 nm)。可以推测，在聚集的HEM中发生了激子相互作用，这是由于UVB照射后的血红蛋白变性引起的。此外，聚集过程中拉曼标记谱带的变化主要源于激子相互作用。在整个过程中，较高的UVB辐射剂量会对血红蛋白造成更大的损害。
Confocal micro-Raman spectroscopy is employed to monitor the damage to haemoglobin from mid-ultraviolet (UVB) radiation. We obtained the Raman spectra of an erythrocyte, which indicated that a peroxidation reaction occurs after UVB radiation. Further, the surface enhanced Raman scattering (SERS) spectra of isolated haemoglobin show that the intensities of the 1375 and 1399 cm(-1) bands, which are markers of haem aggregation, obviously increase with prolonged UVB irradiation. This increase reveals that haem aggregation occurs in the peroxidation of erythrocytes. The UV-Vis spectra of isolated haemoglobin indicate that the Soret band, which is indicative of excitonic interactions in the aggregated haems, has a redshift (similar to 12 nm) after 30 min of UVB irradiation of erythrocytes. It can be deduced that an excitonic interaction occurs in the aggregated haems, which is caused by haemoglobin denaturation following UVB irradiation. In addition, the changes of the Raman marker bands during aggregation primarily originate from excitonic interactions. Throughout the process, a higher UVB radiation dose causes greater damage to haemoglobin.