Dual RNA-seq of Xanthomonas oryzae pv. oryzicola infecting rice reveals novel insights into bacterial-plant interaction
Dual RNA-seq of Xanthomonas oryzae pv. oryzicola infecting rice reveals novel insights into bacterial-plant interaction
复制标题
米黄单胞菌 pv. 的双 RNA 测序。
DOI：
10.1371/journal.pone.0215039
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发表时间：
2019-04-17
期刊：
影响因子：
3.7
通讯作者：
Huang, Sheng
中科院分区：
文献类型：
--
作者：
Liao, Zhou-Xiang;Ni, Zhe;Huang, Sheng
革兰氏阴性菌黄单胞菌黄单胞菌致病型（Xanthomonasplantpv.）水稻细菌性条斑病（BLS）是水稻上最具破坏性的病害之一，由oryzicola（Xoc）引起。那是重要的主食作物。Xoc可以通过气孔和伤口侵入寄主叶片，其第三型分泌系统（T3 SS）是其致病生活方式的关键。在这项研究中，使用一种新的双重RNA-seq方法，我们检查了野生型Xoc GX 01及其T3 SS缺陷株（T3 SD）接种样品中的水稻和Xoc的转录组，以研究两种生物体的整体转录变化。与T3 SD株系相比，野生型Xoc GX 01接种水稻后，一系列与植物防御相关的基因表达发生了显著变化，包括植物信号传导途径中的基因表达改变，苯丙氨酸代谢、类黄酮和苦内酯生物合成下调，表明植物防御反应受到抑制，胼胝质沉积和植物防御素积累减少。此外，一些已知的转录激活因子样效应物（TALE）靶标被Xoc GX 01诱导，例如OsSult 3; 6，其有助于水稻易感性。GX 01还诱导了一些与细胞伸长相关的基因，包括几个expansin基因，表明Xoc可能利用这一途径削弱细胞壁强度，有利于细菌感染。另一方面，与野生型相比，T3 SD菌株在植物体内的转录组表现为ATP、蛋白质和多糖合成的下调，以及抗氧化和解毒相关基因的上调，表明T3 SD菌株在植物体内面临严重的饥饿和氧化胁迫，缺乏功能性T3 SS。此外，比较全球转录谱XOC在植物和介质中显示了上调的毒力因子的合成和分泌在植物中有利于细菌感染。总的来说，这项研究提供了一个全面的代表性的主机和细菌病原体之间的串扰，揭示了Xoc水稻致病动态的见解，并揭示了这种重要的病原体所利用的新策略，导致疾病。
The Gram-negative bacterium Xanthomonas oryzae pv. oryzicola (Xoc) is the causal agent of rice bacterial leaf streak (BLS), one of the most destructive diseases of rice (Oryza sativa L.) that is the important staple crop. Xoc can invade host leaves via stomata and wounds and its type three secretion system (T3SS) is pivotal to its pathogenic lifestyle. In this study, using a novel dual RNA-seq approach, we examined transcriptomes of rice and Xoc in samples inoculated with wild type Xoc GX01 and its T3SS defective strain (T3SD), to investigate the global transcriptional changes in both organisms. Compared with T3SD strain, rice inoculated with wild type Xoc GX01 resulted in significant expression changes of a series of plant defence related genes, including ones altered in plant signalling pathway, and downregulated in phenylalanine metabolism, flavonoid and momilactone biosynthesis, suggesting repression of plant defence response and reduction in both callose deposition and phytoalexin accumulation. Also, some known transcription activator-like effector (TALE) targets were induced by Xoc GX01, e.g. OsSultr3; 6 which contributes to rice susceptibility. Some cell elongation related genes, including several expansin genes, were induced by GX01 too, suggesting that Xoc may exploit this pathway to weaken cell wall strength, beneficial for bacterial infection. On the other hand, compared with wild type, the T3SD strain transcriptome in planta was characterized by downregulation of ATP, protein and polysaccharide synthesis, and upregulation of antioxidation and detoxification related genes, revealing that T3SD strain faced serious starvation and oxidation stresses in planta without a functional T3SS. In addition, comparative global transcript profiles of Xoc in planta and in medium revealed an upregulation of virulence factor synthesis and secretion in planta in favour of bacterial infection. Collectively, this study provides a comprehensive representation of cross talk between the host and bacterial pathogen, revealing insights into the Xoc-rice pathogenic dynamic and reveals novel strategies exploited by this important pathogen to cause disease.