Pregnant mouse corpora lutea: immunocytochemical localization of relaxin and ultrastructure.
Pregnant mouse corpora lutea: immunocytochemical localization of relaxin and ultrastructure.
复制标题
怀孕小鼠黄体：松弛素和超微结构的免疫细胞化学定位。
DOI：
10.1095/biolreprod31.2.391
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发表时间：
1984
影响因子：
3.6
通讯作者：
Sherwood,OD
中科院分区：
文献类型：
--
作者：
Anderson,MB;Vaupel,MR;Sherwood,OD
应用非标记抗体过氧化物酶-抗过氧化物酶技术和高度特异的兔抗大鼠松弛素血清，将松弛素定位于妊娠小鼠卵巢黄体。在妊娠第10天，松弛素免疫染色首次出现在黄体周围的黄体细胞中。松弛素免疫染色的细胞数量和染色强度逐渐增加，在妊娠16-18天达到最大。在产后第1天，少数黄体细胞被特异地染色为松弛素，而在产后第2天，黄体细胞没有被染色。对怀孕小鼠卵巢的黄体细胞进行的超微结构研究显示，存在一个明显的电子致密、膜结合的颗粒群，这是在怀孕第12天首次观察到的。颗粒数量增加，在妊娠16至18天达到最大值，产后第2天消失。该颗粒群的出现和消失与黄体细胞松弛素免疫染色密切相关。我们认为，这些颗粒可能是松弛素在妊娠小鼠卵巢中储存的亚细胞位置。
Relaxin was localized in corpora lutea of pregnant mouse ovaries by using the unlabeled antibody peroxidase-antiperoxidase technique and a highly specific rabbit antirat relaxin serum. Relaxin immunostaining was first observed in luteal cells located at the periphery of corpora lutea on Day 10 of gestation. The number of relaxin immunostained cells and the intensity of the stain gradually increased to reach a maximum between Days 16 and 18 of gestation. While a few luteal cells were specifically stained for relaxin on Day 1 postpartum, no luteal cells were stained on Day 2 postpartum.Ultrastructural studies of luteal cells from pregnant mouse ovaries revealed the presence of a distinct electron-dense, membrane-bound granule population, which was first observed on Day 12 of gestation. The granules increased in number to reach a maximum between Days 16 and 18 of gestation, and were absent by Day 2 postpartum. The appearance and disappearance of this granule population closely paralleled the relaxin immunostaining in the luteal cells. We suggest that the granules may be the subcellular sites of relaxin storage in the pregnant mouse ovary.