Expression of adipogenic transcription factors in adipose tissue of fattening Wagyu and Holstein steers
Expression of adipogenic transcription factors in adipose tissue of fattening Wagyu and Holstein steers
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DOI：
10.1016/j.meatsci.2008.06.022
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发表时间：
2009-01-01
期刊：
影响因子：
7.1
通讯作者：
Nakanishi, N.
中科院分区：
文献类型：
--
作者：
Yamada, T.;Kawakami, S. -I;Nakanishi, N.
在本试验中，我们研究了品种差异对不同育肥期（19月龄和24月龄）日本黑牛和荷兰黑牛脂肪组织中不同解剖部位（皮下、肌间和肠系膜）脂肪生成转录因子C/EBP家族（C/EBP α、C/EBP β - lap、C/EBP β - lip和C/EBP delta）和PPAR γ蛋白表达的影响。19月龄和牛肠系膜脂肪组织中C/EBP β - lap和C/EBP α的表达量显著高于荷斯坦猪。19月龄和牛皮下、肌间和肠系膜脂肪组织中C/EBP6的表达显著高于荷斯坦猪。19月龄和牛的血浆胰岛素浓度有高于荷斯坦牛的趋势。24月龄时，不同品种的成脂转录因子表达和血浆胰岛素浓度无显著差异。上述结果表明，育肥和牛和荷斯坦阉牛在19月龄时C/EBP家族基因表达存在品种差异，而在24月龄前，品种差异可能消失。(c) 2008 Elsevier Ltd.版权所有。
In this experiment, we studied the effects of breed differences on the protein expression of adipogenic transcription factors, the C/EBP family (C/EBP alpha, C/EBP beta-LAP, C/EBP beta-LIP and C/EBP delta) and PPAR gamma, in the adipose tissues of Japanese Black (Wagyu) and Holstein steers from various anatomical sites (subcutaneous, intermuscular, and mesenteric) at different fattening periods (19 and 24 months of age). The expression of C/EBP beta-LAP and C/EBP alpha in the mesenteric fat tissue of Wagyu at 19 months of age was significantly higher than that of Holstein. The expression of C/EBP6 in the subcutaneous, intermuscular and mesenteric fat tissue of Wagyu at 19 months of age was significantly higher than that of Holstein. The plasma insulin concentrations of Wagyu steers at 19 months of age tended to be higher than those of Holstein. No significant differences in the expression of the adipogenic transcription factors and plasma insulin concentration were observed between the breeds at 24 months of age. These results suggest the existence of breed difference on the expression of the C/EBP family between fattening Wagyu and Holstein steers at 19 months of age, whereas breed difference might have disappeared before 24 months of age. (c) 2008 Elsevier Ltd. All rights reserved.