Mechanism of bioactive molecular extraction from mineralized dentin by calcium hydroxide and tricalcium silicate cement
Mechanism of bioactive molecular extraction from mineralized dentin by calcium hydroxide and tricalcium silicate cement
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氢氧化钙和硅酸三钙水泥从矿化牙本质中提取生物活性分子的机理。
DOI：
10.1016/j.dental.2017.11.010
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发表时间：
2018-02-01
期刊：
影响因子：
5
通讯作者：
Niu, Li-na
中科院分区：
文献类型：
--
作者：
Huang, Xue-qing;Camba, John;Niu, Li-na
目标.本研究的目的是阐明氢氧化钙（Ca（OH）（2））和硅酸三钙水泥（TSC）从矿化牙本质中提取生物活性分子的机制。方法和结果。透射电子显微镜用于提供在用Ca（OH）（2）或一组水合TSC覆盖1-3个月的牙本质表面中胶原降解的证据。在牙本质表面观察到一个1微米厚的胶原降解区。傅立叶变换红外光谱法用于识别暴露于Ca（OH）（2）的牙本质中磷灰石/胶原比例的增加。使用三点弯曲，暴露于Ca（OH）（2）的牙本质表现出显著的弯曲强度降低。使用尺寸排阻色谱法，发现源自Ca（OH）（2）的小尺寸的氢氧根离子使这些离子能够渗透矿化胶原的纤维内隔室并降解胶原纤维而不影响磷灰石矿物。使用ELISA，发现TGF-β 1从用Ca（OH）（2）覆盖3个月的牙本质中提取。与溶解牙本质的矿物质成分以释放生物活性分子的酸不同，碱性材料如Ca（OH）（2）或TSC通过胶原降解释放生长因子如TGF-β 1。Ca（OH）（2）和TSC的生物活性分子提取能力使得这些牙科材料对于盖髓和牙髓再生是优异的。然而，这些非常理想的性质似乎与矿化牙本质内胶原基质降解的不利影响交织在一起，导致弯曲强度降低。(C)2017年牙科材料学院。由爱思唯尔有限公司出版。保留所有权利。
Objectives. The objective of the present study was to elucidate the mechanism of bioactive molecule extraction from mineralized dentin by calcium hydroxide (Ca(OH)(2)) and tricalcium silicate cements (TSC).Methods and results. Transmission electron microscopy was used to provide evidence for collagen degradation in dentin surfaces covered with Ca(OH)(2) or a set, hydrated TSC for 1-3 months. A one micron thick collagen degradation zone was observed on the dentin surface. Fourier transform-infrared spectroscopy was used to identify increases in apatite/collagen ratio in dentin exposed to Ca(OH)(2). Using three-point bending, dentin exposed to Ca(OH)(2) exhibited significant reduction in flexural strength. Using size exclusion chromatography, it was found that the small size of the hydroxyl ions derived from Ca(OH)(2) enabled those ions to infiltrate the intrafibrillar compartment of mineralized collagen and degrade the collagen fibrils without affecting the apatite minerals. Using ELISA, TGF-beta 1 was found to be extracted from dentin covered with Ca(OH)(2) for 3 months. Unlike acids that dissolve the mineral component of dentin to release bioactive molecules, alkaline materials such as Ca(OH)(2) or TSC released growth factors such as TGF-beta 1 via collagen degradation.Significance. The bioactive molecule extraction capacities of Ca(OH)(2) and TSC render these dental materials excellent for pulp capping and endodontic regeneration. These highly desirable properties, however, appear to be intertwined with the untoward effect of degradation of the collagen matrix within mineralized dentin, resulting in reduced flexural strength. (C) 2017 The Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.