Testing of antibiotic releasing implant coatings to fight bacteria in combat-associated osteomyelitis - an in-vitro study
Testing of antibiotic releasing implant coatings to fight bacteria in combat-associated osteomyelitis - an in-vitro study
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DOI：
10.1007/s00264-016-3142-2
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发表时间：
2016-05-01
影响因子：
2.7
通讯作者：
Wildemann, Britt
中科院分区：
文献类型：
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作者：
Back, David Alexander;Bormann, Nicole;Wildemann, Britt
目的 局部应用抗生素可以支持预防创伤后骨髓炎的外科手术。这项体外研究调查了植入物涂层中抗生素的释放和对与战斗相关骨髓炎相关细菌的影响。方法克氏针涂有不同浓度的聚（D，L-丙交酯）和环丙沙星、庆大霉素、粘菌素、达托霉素或头孢西丁。对不同革兰氏阳性或革兰氏阴性细菌的释放进行了量化，并单独和组合测试了抗菌活性。为了排除毒性作用，将原代成骨细胞样细胞暴露于不同浓度的抗生素涂层中。结果除头孢西丁外，所有抗生素单独使用和联合使用均显示出初始爆发释放，具有剂量依赖性抗菌活性，并且对成骨细胞样细胞没有负面影响。结论植入物涂层可以定制单层或双层抗生素涂层，以有效对抗不同细菌以及治疗战斗获得性骨髓炎中的混合感染。然而，必须考虑各个抗生素的最佳载药量和降解行为。
Purpose Surgical procedures to prevent osteomyelitis after trauma can be supported by local application of antibiotics. This in-vitro study investigated the release and impact of antibiotics from implant coatings against bacteria associated with combat-related osteomyelitis.Methods K-wires were coated with poly(D, L-lactide) and ciprofloxacin, gentamicin, colistin, daptomycin or cefoxitin in different concentrations. The release was quantified and antimicrobial activity tested for different gram-positive or gram-negative bacteria, alone and in combination. To exclude toxic effects, primary osteoblast-like cells were exposed to antibiotic coating concentrations.Results All antibiotics alone and in combination showed an initial burst release with dose dependent antimicrobial activity and no negative effects on osteoblast-like cells, except for cefoxitin.Conclusions Implant coatings can be customized with single or double antibiotic coatings to effectively fight different bacteria and also mixed infections in the treatment of a combat-acquired osteomyelitis. However, optimal drug load and degradation behaviour of individual antibiotics have to be considered.