Synthesis of Next-Generation Maleimide Radical Labels
Synthesis of Next-Generation Maleimide Radical Labels
复制标题
下一代马来酰亚胺自由基标记的合成
作者：
Lovett J
介绍了四种新的含氮氧自由基的新一代马来酰亚胺的合成与表征。每个新的标记具有单个离去基团，其为苯氧基或溴。马来酰亚胺和含氮氧自由基框架之间的连接基是短链或非手性较长链的外消旋混合物。这些分子已被设计用于位点特异性标记蛋白质中的邻位半胱氨酸以用于磁共振研究。最终产物的表征包括结晶学和抹香鲸肌红蛋白的标记。
The synthesis and characterization of four new nitroxide-radical-containing next-generation maleimides are presented. Each new label has a single leaving group which is either a phenoxyl or bromide. The linker between the maleimide and the nitroxide-containing framework is either a racemic mixture of a short chain or an achiral longer chain. These molecules have been designed to site-specifically label vicinal cysteines in proteins for magnetic resonance studies. The characterization of the final products includes crystallography and the labeling of sperm whale myoglobin protein.