Anti-influenza H7 human antibody targets antigenic site in hemagglutinin head domain interface
Anti-influenza H7 human antibody targets antigenic site in hemagglutinin head domain interface
复制标题
DOI：
10.1172/jci136032
复制
发表时间：
2020-09-01
影响因子：
15.9
通讯作者：
Crowe, James E., Jr.
中科院分区：
文献类型：
--
作者：
Dong, Jinhui;Gilchuk, Iuliia;Crowe, James E., Jr.
尽管针对甲型流感病毒血凝素 (HA) 的广泛保护性干靶向抗体已得到充分研究，但有关广泛识别头域的抗体的信息非常有限。我们确定了禽 H7N9 流感病毒的 HA 蛋白与泛 H7、非中和性、保护性人类抗体复合物的晶体结构。该结构揭示了 HA 头域三聚体界面 (TI) 中的 B 细胞表位。第二个主要保护性 TI 表位的发现支持了一种模型，其中未切割的 HA 三聚体以高度动态的状态存在于受感染细胞的表面，暴露了隐藏的 HA 头域特征。
Although broadly protective, stem-targeted Abs against the influenza A virus hemagglutinin (HA) have been well studied, very limited information is available on Abs that broadly recognize the head domain. We determined the crystal structure of the HA protein of the avian H7N9 influenza virus in complex with a pan-H7, non-neutralizing, protective human Ab. The structure revealed a B cell epitope in the HA head domain trimer interface (TI). This discovery of a second major protective TI epitope supports a model in which uncleaved HA trimers exist on the surface of infected cells in a highly dynamic state that exposes hidden HA head domain features.