Complex interactions in EML cell stimulation by stem cell factor and IL-3.
Complex interactions in EML cell stimulation by stem cell factor and IL-3.
复制标题
干细胞因子和 IL-3 在 EML 细胞刺激中的复杂相互作用。
DOI：
10.1073/pnas.1018002108
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发表时间：
2011
影响因子：
11.1
通讯作者：
Weissman,ShermanM
中科院分区：
文献类型：
--
作者：
Ye,Zhi-jia;Gulcicek,Erol;Stone,Kathryn;Lam,Tukiet;Schulz,Vincent;Weissman,ShermanM
红系髓样淋巴（EML）细胞是已建立的多能造血前体细胞系，其可在包含干细胞因子（SCF）的培养基中维持。EML培养物含有细胞的异质混合物，包括在SCF中快速繁殖并可克隆再生混合群体的谱系阴性的CD 34+细胞亚群。EML细胞的第二主要亚群由谱系阴性组成。CD 34-细胞可以在IL-3中增殖，但在SCF中生长缓慢，即使它们表达SCF受体（c-kit）。SCF协同刺激这些细胞对IL-3的反应，我们提出的证据表明，协同作用和c-kit反应的抑制可能是通过与IL-3受体的直接相互作用介导的。此外，通过单独的任一细胞因子的各种底物的酪氨酸磷酸化的相对水平不同于通过两种细胞因子的组合产生的酪氨酸磷酸化的相对水平，这表明通过两种细胞因子的组合产生的细胞信号传导不同于通过单独的任一细胞因子产生的细胞信号传导。
Erythroid myeloid lymphoid (EML) cells are an established multipotent hematopoietic precursor cell line that can be maintained in medium including stem cell factor (SCF). EML cultures contain a heterogeneous mixture of cells, including a lineage-negative, CD34+subset of cells that propagate rapidly in SCF and can clonally regenerate the mixed population. A second major subset of EML cells consists of lineage-negative. CD34−cells that can be propagated in IL-3 but grow slowly, if at all, in SCF, although they express the SCF receptor (c-kit). The response of these cells to IL-3 is stimulated synergistically by SCF, and we present evidence that both the synergy and the inhibition of c-kit responses may be mediated by direct interaction with IL-3 receptor. Further, the relative level of tyrosine phosphorylation of various substrates by either cytokine alone differs from that produced by the combination of the two cytokines, suggesting that cell signaling by the combination of the two cytokines differs from that produced by either alone.