A genetic pathway for tapetum development and function in Arabidopsis.
A genetic pathway for tapetum development and function in Arabidopsis.
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DOI：
10.1111/j.1744-7909.2011.01078.x
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发表时间：
2011-11
影响因子：
11.4
通讯作者：
Jun Zhu;Y. Lou;Xiaofeng Xu;Zhongnan Yang
中科院分区：
文献类型：
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作者：
Jun Zhu;Y. Lou;Xiaofeng Xu;Zhongnan Yang
在花药发育过程中，绒毡层细胞参与复杂的过程，包括有丝分裂和细胞凋亡（程序性细胞死亡）。绒毡层提供花粉发育所必需的许多蛋白质、脂类、多糖和其他分子。已经报道了几个转录因子，包括DYT1、TDF1、AMS、MS188和MS1，在拟南芥绒毡层发育和功能中是必需的。在这里，我们提出了一个详细的细胞学分析敲除这些基因的突变体，沿着与原位RNA杂交实验和双突变体分析表明，这些转录因子形成的遗传途径绒毡层发育。DYT1、TDF1和AMS在绒毡层的早期发育中起作用，而MS 188和MS 1在绒毡层的晚期发育中起重要作用。本研究揭示的绒毡层发育的遗传途径有助于进一步研究绒毡层发育的功能和分子机制。
In anther development, tapetal cells take part in complex processes, including endomitosis and apoptosis (programmed cell death). The tapetum provides many of the proteins, lipids, polysaccharides and other molecules necessary for pollen development. Several transcription factors, including DYT1, TDF1, AMS, MS188 and MS1, have been reported to be essential for tapetum development and function in Arabidopsis thaliana. Here, we present a detailed cytological analysis of knockout mutants for these genes, along with an in situ RNA hybridization experiment and double mutant analysis showing that these transcription factors form a genetic pathway in tapetum development. DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. The genetic pathway revealed in this work facilitates further investigation of the function and molecular mechanisms of tapetum development in Arabidopsis.