Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4
Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4
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DOI：
10.1016/s0092-8674(03)00036-9
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发表时间：
2003-02-07
期刊：
影响因子：
64.5
通讯作者：
Staskawicz, BJ
中科院分区：
文献类型：
--
作者：
Axtell, MJ;Staskawicz, BJ
植物已经发展出一种复杂的先天免疫系统，以识别入侵的病原体并诱导一系列宿主防御机制，从而产生抗病性。病原体识别通常是由植物性疾病耐药性（R）蛋白专门反应于一种或几个病原体衍生的分子的介导的。这种特异性导致了R蛋白功能的受体配体模式的建议。丁香假单胞菌递送细菌效应蛋白AVRRPT2在表达RPS2 R蛋白的拟南芥植物中特异性诱导了抗病性。我们证明RPS2与拟南芥RIN4物理相互作用，并且AVRRPT2在激活RPS2途径期间导致RIN4消除。 AVRRPT2介导的RIN4消除也发生在RPS2，NDR1和ATRAR1突变体背景中，表明可以独立于RPS2介导的信号传导途径来实现此活性。因此，我们建议RPS2基于对RIN4消失的感知而不是直接识别AVRRPT2的信号传导。
Plants have evolved a sophisticated innate immune system to recognize invading pathogens and to induce a set of host defense mechanisms resulting in disease resistance. Pathogen recognition is often mediated by plant disease resistance (R) proteins that respond specifically to one or a few pathogen-derived molecules. This specificity has led to suggestions of a receptor-ligand mode of R protein function. Delivery of the bacterial effector protein AvrRpt2 by Pseudomonas syringae specifically induces disease resistance in Arabidopsis plants expressing the RPS2 R protein. We demonstrate that RPS2 physically interacts with Arabidopsis RIN4 and that AvrRpt2 causes the elimination of RIN4 during activation of the RPS2 pathway. AvrRpt2-mediated RIN4 elimination also occurs in the rps2, ndr1, and Atrar1 mutant backgrounds, demonstrating that this activity can be achieved independent of an RPS2-mediated signaling pathway. Therefore, we suggest that RPS2 initiates signaling based upon perception of RIN4 disappearance rather than direct recognition of AvrRpt2.