Cannabinoid Disposition in Oral Fluid after Controlled Smoked Cannabis
Cannabinoid Disposition in Oral Fluid after Controlled Smoked Cannabis
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DOI：
10.1373/clinchem.2011.177881
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发表时间：
2012-04-01
影响因子：
9.3
通讯作者：
Huestis, Marilyn A.
中科院分区：
文献类型：
--
作者：
Lee, Dayong;Schwope, David M.;Huestis, Marilyn A.
背景：我们测量了受控吸食大麻后口服液中的Delta(9)-四氢大麻酚(THC)、11-Nor-9-Carxy-THC(THCCOOH)、大麻二酚(CBD)和大麻酚(CBN)的处置，以评估监测多种大麻类化合物是否有助于改善测试解释。Of是在随意吸烟一支6.8%THC香烟后用Quantisal(TM)设备收集的。用二维GC-MS对大麻素类化合物进行了定量。结果：10名参与者提供了86份样本--开始吸烟前0.5h和开始吸烟后0.25、0.5、1、2、3、4、6和22h。吸烟前，4名和9名受试者的THC和THCCOOH分别为阳性，而CBD和CBN均为阴性。THC、CBD和CBN的最大浓度出现在0.5h内，中位数分别为644、30.4和49.0mg/L。所有样本在6h均为阳性(2.1~44.4Mu g/L)，其中4例在22h呈阳性，其中3例(0.6~2.1mg/L)和4例(1.0~4.4Mu g/L)仅在6h内阳性。最大三氯甲烷浓度的中位数为115 ng/L，6h内全部阳性(14.8~263 ng/L)，22h时6个样本中有5个阳性。结论：通过对多种大麻类化合物的定量和对吸食大麻后不同分析界值的评估，我们确定了药物检测窗口，找到了近期吸烟的建议标志物，最大限度地减少了被动污染的可能性。(C)2011年美国临床化学协会
BACKGROUND: We measured Delta(9)-tetrahydrocannabinol (THC), 11-nor-9-carboxy-THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) disposition in oral fluid (OF) following controlled cannabis smoking to evaluate whether monitoring multiple cannabinoids in OF improved OF test interpretation.METHODS: Cannabis smokers provided written informed consent for this institutional review board-approved study. OF was collected with the Quantisal (TM) device following ad libitum smoking of one 6.8% THC cigarette. Cannabinoids were quantified by 2-dimensional GC-MS. We evaluated 8 alternative cutoffs based on different drug testing program needs.RESULTS: 10 participants provided 86 OF samples -0.5 h before and 0.25, 0.5, 1, 2, 3, 4, 6, and 22 h after initiation of smoking. Before smoking, OF samples of 4 and 9 participants were positive for THC and THCCOOH, respectively, but none were positive for CBD and CBN. Maximum THC, CBD, and CBN concentrations occurred within 0.5 h, with medians of 644, 30.4, and 49.0 mu g/L, respectively. All samples were THC positive at 6 h (2.1-44.4 mu g/L), and 4 of 6 were positive at 22 h. CBD and CBN were positive only up to 6 h in 3 (0.6-2.1 mu g/L) and 4 (1.0-4.4 mu g/L) participants, respectively. The median maximum THCCOOH OF concentration was 115 ng/L, with all samples positive to 6 h (14.8-263 ng/L) and 5 of 6 positive at 22 h.CONCLUSIONS: By quantifying multiple cannabinoids and evaluating different analytical cutoffs after controlled cannabis smoking, we determined windows of drug detection, found suggested markers of recent smoking, and minimized the potential for passive contamination. (C) 2011 American Association for Clinical Chemistry