Validation of tissue microarray technology in breast carcinoma
Validation of tissue microarray technology in breast carcinoma
复制标题
DOI：
10.1038/labinvest.3780204
复制
发表时间：
2000-12-01
影响因子：
5
通讯作者：
Rimm, DL
中科院分区：
文献类型：
--
作者：
Camp, RL;Charette, LA;Rimm, DL
组织微阵列技术的最新发展促进了大规模的回顾性队列研究，这些研究使用档案福尔马林固定、石蜡包埋的组织。广泛接受微阵列的一个主要障碍是，它们减少了从整个组织切片到直径0.6毫米的圆盘的组织分析量，这可能不能代表整个肿瘤的蛋白质表达模式。在这项研究中，我们在38例浸润性乳腺癌中检测了足以代表浸润性乳腺癌中三种常见抗原（雌激素受体、孕激素受体和Her2/neu癌基因）表达的磁盘数。我们比较了2到10个微阵列磁盘和整个组织切片的染色，并确定在95%以上的病例中，两个磁盘的分析与整个组织切片的分析相当。为了评估在这种阵列中使用档案组织的潜力，我们创建了一个乳腺癌微阵列，从1932年到现在，每十年有8到11个病例，并评估了这些标记物和其他标记物的抗原性。该阵列表明许多蛋白质保持其抗原性超过60年，从而验证了他们在档案组织上的研究。我们得出结论，具有2倍冗余的组织微阵列技术是分析大型档案队列中蛋白质表达的一种有价值且准确的方法。
The recent development of tissue microarray technology has potentiated large-scale retrospective cohort studies using archival formalin-fixed, paraffin-embedded tissues. A major obstacle to broad acceptance of microarrays is that they reduce the amount of tissue analyzed from a whole tissue section to a disk, 0.6 mm in diameter, that may not be representative of the protein expression patterns of the entire tumor. In this study, we examine the number to disks required to adequately represent the expression of three common antigens in invasive breast carcinoma-estrogen receptor, progesterone receptor, and the Her2/neu oncogene-in 38 cases of invasive breast carcinoma. We compared the staining of 2 to 10 microarray disks and the whole tissue sections from which they were derived and determined that analysis of two disks is comparable to analysis of a whole tissue section in more than 95% of cases. To evaluate the potential for using archival tissue in such arrays, we created a breast cancer microarray of 8 to 11 cases from each decade beginning in 1932 to the present day and evaluated the antigenicity of these markers and others. This array demonstrates that many proteins retain their antigenicity for more than 60 years, thus validating their study on archival tissues. We conclude that the tissue microarray technique, with 2-fold redundancy, is a valuable and accurate method for analysis of protein expression in large archival cohorts.