Polymorphisms of DNA repair gene XRCC3 Thr241Met and risk of gastric cancer in a Chinese population
Polymorphisms of DNA repair gene XRCC3 Thr241Met and risk of gastric cancer in a Chinese population
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DNA修复基因XRCC3 Thr241Met多态性与中国人群胃癌风险
DOI：
10.1016/j.canlet.2003.09.003
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发表时间：
2004-03-31
期刊：
影响因子：
9.7
通讯作者：
Wei, QY
中科院分区：
文献类型：
--
作者：
Shen, HB;Wang, XR;Wei, QY
哺乳动物细胞不断暴露于多种内源性和外源性的基因毒性剂。 DNA 修复的遗传变异可能会增加人类患癌症的风险。在这项中国人群病例对照研究中，我们检验了 DNA 修复基因 XRCC3（X 射线修复交叉互补组 3）的 C 至 T 变体 (Thr241Met) 与患胃癌风险相关的假设。我们使用聚合酶链反应限制性片段长度多态性 (PCR-RFLP) 对 188 名经组织学证实的胃癌患者和 166 名频率匹配的无癌对照患者进行了该变异的基因分型。 XRCC3 基因型和等位基因频率在病例和对照之间没有显着差异（基因型 P = 0.99；等位基因 P = 0.76）。健康中国对照者的 XRCC3 24 1 Met 等位基因频率 (4.8%) 显着低于先前报道的健康美国白种人对照者 (38.9%)。与XRCC3 241Thr/Thr基因型相比，变异XRCC3241Thr/Met和Met/Met基因型与胃癌风险增加无关（调整优势比（ORa），1.06；95%置信区间（CI），0.52-2.16）。这些发现表明 XRCC3 Thr241Met 的多态性可能在胃癌的病因学中不起作用。需要对更多受试者进行进一步研究，并同时测量同一途径中 DNA 修复基因的不同多态性，以证实这些发现。 (C) 2003 Elsevier Ireland Ltd. 保留所有权利。
Mammalian cells are constantly exposed to a wide variety of genotoxic agents from both endogenous and exogenous sources. Genetic variability in DNA repair may contribute to human cancer risk. In this population-based case-control study in China, we tested the hypothesis that a C to T variant (Thr241Met) of DNA repair gene XRCC3 (X-ray repair cross-complementing group 3) is associated with risk of developing gastric cancer. We genotyped for this variant using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) in 188 histologically confirmed gastric cancer patients and 166 frequency-matched cancer-free controls. The XRCC3 genotype and allele frequencies were not significantly different between cases and controls (P = 0.99 for genotype; P = 0.76 for allele). The XRCC3 24 1 Met allele frequency (4.8%) was significantly lower in healthy Chinese controls than previously reported healthy US Caucasian controls (38.9%). Compared with the XRCC3 241Thr/Thr genotype, the variant XRCC3241Thr/Met and Met/Met genotypes were not associated with an increased risk of gastric cancer (adjusted odds ratio (ORa), 1.06; 95% confidence interval (CI), 0.52-2.16). These findings suggest that polymorphisms of XRCC3 Thr241Met may not play a role in the etiology of gastric cancer. Further studies with a larger number of subjects and simultaneous measurement of different polymorphisms in DNA repair genes in the same pathway are needed to confirm these findings. (C) 2003 Elsevier Ireland Ltd. All rights reserved.