Intestinal lengthening in an innovative rodent surgical model
Intestinal lengthening in an innovative rodent surgical model
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DOI：
10.1016/j.jpedsurg.2014.09.022
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发表时间：
2014-12-01
影响因子：
2.4
通讯作者：
Dunn, James C. Y.
中科院分区：
文献类型：
--
作者：
Sullins, Veronica F.;Scott, Andrew;Dunn, James C. Y.
目的：目前的机械延长动物模型将肠段与肠连续性分开。这样的模型难以用于重复的延长手术，并且在恢复连续性期间导致肠组织损失。我们试图建立一种新的手术模型，以允许多个延长程序的目的，最大限度地增加组织后肠lengthening.Methods：一个Roux-en-y空肠空肠吻合术与6厘米盲端Roux肢体在近端空肠的大鼠。将封装的1 cm聚己内酯弹簧放入Roux分支的闭合端，并用血管环固定。结果：空肠段由1.0cm延长至3.0cm，空肠段长度由1.0cm延长至3.0cm，空肠段长度由1.0cm延长至3.0cm。与正常肠相比，延长的肠段平滑肌厚度增加，粘膜下神经节减少，肌间神经节数量相似。当与正常空肠粘膜相比，延长段表现出不变的绒毛高度和增加隐窝depth.Conclusions：我们创造了一个创新的手术模型肠延长和成功延长空肠段与可降解弹簧。Roux-en-y模型可以允许使用可降解弹簧来治疗短肠综合征。(C)2014 Elsevier Inc. All rights reserved.
Purpose: Current animal models of mechanical lengthening separate intestinal segments from enteric continuity. Such models are difficult to use for repeated lengthening procedures and result in intestinal tissue loss during restoration into continuity. We sought to create a novel surgical model to allow multiple lengthening procedures for the purpose of maximizing the net increase in tissue after intestinal lengthening.Methods: A Roux-en-y jejunojejunostomy with a 6-cm blind-ended Roux limb was created in the proximal jejunum of rats. Encapsulated 1-cm polycaprolactone springs were placed into the closed end of the roux limb and secured with a vessel loop. After 4 weeks, lengthened segments and normal jejunum were retrieved for histologic analysis.Results: Jejunal segments were lengthened from 1.0 cm to 3.0 cm. Lengthened segments had increased smooth muscle thickness, fewer submucosal ganglia, and similar numbers of myenteric ganglia compared to normal intestine. When compared to normal jejunal mucosa, lengthened segments demonstrated unchanged villus height and increased crypt depth.Conclusions: We created an innovative surgical model for intestinal lengthening and successfully lengthened jejunal segments with a degradable spring. The Roux-en-y model may allow the use of a degradable spring for the treatment of short bowel syndrome. (C) 2014 Elsevier Inc. All rights reserved.