APOLIPOPROTEIN-E SYNTHESIS IN HUMAN-KIDNEY, ADRENAL-GLAND, AND LIVER
APOLIPOPROTEIN-E SYNTHESIS IN HUMAN-KIDNEY, ADRENAL-GLAND, AND LIVER
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DOI：
10.1073/pnas.80.1.283
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BLUM, CB
中科院分区：
文献类型：
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作者：
BLUE, ML;WILLIAMS, DL;BLUM, CB
将人组织与放射性标记的氨基酸在体外孵育，以确定血浆载脂蛋白是否在人肾脏中合成。随后，用抗载脂蛋白[apo] E、apo B、apo AI和高密度脂蛋白（HDL）的散装载脂蛋白的抗血清筛选组织提取物。在肾脏和肾上腺皮质中发现了新合成的载脂蛋白E，但未发现载脂蛋白AI或B。体外apo E合成的相对速率估计表明，肾上腺和肾脏蛋白质合成的相当大一部分致力于apo E合成。肾皮质apoE合成的相对速率比肾髓质高4-6倍。免疫反应性apo E的分析表明，肾脏和肾上腺apo E种类在十二烷基硫酸钠/聚丙烯酰胺凝胶中具有与血浆apo E相同的电泳迁移率。通过高分辨率二维凝胶分析的进一步表征表明，新合成的肾脏和肾上腺apo E的亚型对应于血浆apo E的特异性亚型。来自外周组织的载脂蛋白可能在脂质转运和代谢中起重要作用。
Human tissues were incubated in vitro with radiolabeled amino acids to determine whether plasma apolipoproteins are synthesized in human kidney. Subsequently, tissue extracts were screened with antisera directed against apolipoprotein [apo] E, apo B, apo AI, and bulk apoliproproteins of high density lipoprotein (HDL). Newly synthesized apo E, but not apo AI or B, was identified in kidney and adrenal cortex. Estimates of relative rates of apo E synthesis in vitro suggest that a substantial portion of adrenal and kidney protein synthesis is committed to apo E synthesis. The relative rate of apo E synthesis was 4-6 times greater in kidney cortex than in kidney medulla. Analysis of immunoreactive apo E showed that kidney and adrenal apo E species have the same electrophoretic mobility in sodium dodecyl sulfate/polyacrylamide gels as does plasma apo E. Further characterization by high resolution 2-dimensional gel analysis indicated that the isoforms of newly synthesized kidney and adrenal apo E correspond to specific isoforms of plasma apo E. Apolipoproteins arising from peripheral tissues may play an important role in lipid transport and metabolism.