Soy isoflavone exposure through all life stages accelerates 17β-estradiol-induced mammary tumor onset and growth, yet reduces tumor burden, in ACI rats
Soy isoflavone exposure through all life stages accelerates 17β-estradiol-induced mammary tumor onset and growth, yet reduces tumor burden, in ACI rats
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DOI：
10.1007/s00204-016-1674-2
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发表时间：
2016-08-01
影响因子：
6.1
通讯作者：
Vollmer, Guenter
中科院分区：
文献类型：
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作者：
Moeller, Frank Josef;Pemp, Daniela;Vollmer, Guenter
关于摄入大豆异黄酮（sISO）是否对乳腺癌风险有有益或不利的影响，一直存在争议。因此，我们研究了从怀孕到成年的营养暴露于富含sso的饮食中是否会影响17 β -雌二醇（E2）诱导的大鼠乳腺（MG）癌变。八月-哥本哈根-爱尔兰（ACI）大鼠从受孕到出生后285天一直暴露于膳食sISO。用含E2的硅胶管诱导MG肿瘤发生。每周记录体重、食物摄入量和肿瘤生长情况。尸检时，确定每个肿瘤的数量、位置、大小和重量。血浆样品进行sISO分析，并分析MG的形态。与对照大鼠相比，暴露于sso的大鼠的肿瘤发生率和多样性分别降低了20%和56%。出现肿瘤的时间从25周缩短到20周，暴露于sso的大鼠出现更大的肿瘤。MG肿瘤的组织学表型与所接受的sISO饮食无关，包括粉刺型和筛网型。形态学分析显示，整个安装的mg也没有饮食依赖性差异。终生暴露于sISO降低了ACI大鼠MG癌的总体发病率，尽管发生肿瘤的时间明显缩短。
There is an ongoing debate whether the intake of soy-derived isoflavones (sISO) mediates beneficial or adverse effects with regard to breast cancer risk. Therefore, we investigated whether nutritional exposure to a sISO-enriched diet from conception until adulthood impacts on 17 beta-estradiol (E2)-induced carcinogenesis in the rat mammary gland (MG). August-Copenhagen-Irish (ACI) rats were exposed to dietary sISO from conception until postnatal day 285. Silastic tubes containing E2 were used to induce MG tumorigenesis. Body weight, food intake, and tumor growth were recorded weekly. At necropsy, the number, position, size, and weight of each tumor were determined. Plasma samples underwent sISO analysis, and the morphology of MG was analyzed. Tumor incidence and multiplicity were reduced by 20 and 56 %, respectively, in the sISO-exposed rats compared to the control rats. Time-to-tumor onset was shortened from 25 to 20 weeks, and larger tumors developed in the sISO-exposed rats. The histological phenotype of the MG tumors was independent of the sISO diet received, and it included both comedo and cribriform phenotypes. Morphological analyses of the whole-mounted MGs also showed no diet-dependent differences. Lifelong exposure to sISO reduced the overall incidence of MG carcinomas in ACI rats, although the time-to-tumor was significantly shortened.