The anatomy of microbial cell state transitions in response to oxygen
The anatomy of microbial cell state transitions in response to oxygen
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DOI：
10.1101/gr.6728007
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发表时间：
2007-10-01
期刊：
影响因子：
7
通讯作者：
Baliga, Nitin S.
中科院分区：
文献类型：
--
作者：
Schmid, Amy K.;Reiss, David J.;Baliga, Nitin S.
根据氧气供应量的变化调整生理机能对于所有生物体的生存至关重要。然而，在系统水平上，事件的时间顺序和决定环境氧张力的变化如何以及何时导致适当的细胞反应的调节过程还没有得到很好的理解。因此，我们对嗜盐古菌中的转录组、蛋白质组、ATP 和生长变化进行了分析，以生成一个时间模型，该模型描述了驱动生物体缺氧静止和需氧生长这两种相反细胞状态之间转变的细胞事件。根据该模型，氧气流入后，蛋白质合成的初始爆发先于 ATP 和转录诱导，迅速驱动细胞摆脱缺氧静止状态，最终恢复生长。该模型还表明，静止细胞似乎仍然积极准备从各种不同来源生产能量。对关键基因转录和翻译之间关系的动态时间分析提示了缺氧条件下细胞维持的几种重要机制以及转录后调节的具体实例。
Adjustment of physiology in response to changes in oxygen availability is critical for the survival of all organisms. However, the chronology of events and the regulatory processes that determine how and when changes in environmental oxygen tension result in an appropriate cellular response is not well understood at a systems level. Therefore, transcriptome, proteome, ATP, and growth changes were analyzed in a halophilic archaeon to generate a temporal model that describes the cellular events that drive the transition between the organism's two opposing cell states of anoxic quiescence and aerobic growth. According to this model, upon oxygen influx, an initial burst of protein synthesis precedes ATP and transcription induction, rapidly driving the cell out of anoxic quiescence, culminating in the resumption of growth. This model also suggests that quiescent cells appear to remain actively poised for energy production from a variety of different sources. Dynamic temporal analysis of relationships between transcription and translation of key genes suggests several important mechanisms for cellular sustenance under anoxia as well as specific instances of post- transcriptional regulation.