In vivo evaluation of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin)
In vivo evaluation of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin)
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DOI：
10.1016/s0142-9612(01)00379-9
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发表时间：
2002-06-01
期刊：
影响因子：
14
通讯作者：
Sung, HW
中科院分区：
文献类型：
--
作者：
Chang, Y;Tsai, CC;Sung, HW
在这项研究中，细胞提取过程被用来去除牛心包中的细胞成分，留下了一个主要由不溶性胶原和弹性蛋白组成的框架。文献中假设，这一过程可能会降低材料内的抗原量(或增加生物相容性)。此外，无细胞组织可以为宿主细胞迁移提供一个自然的微环境来再生组织。本研究旨在评价用天然交联剂(京尼平)固定的细胞牛心包和去细胞牛心包皮下植入的生物相容性。此外，还考察了染料木素固定脱细胞组织的组织再生率。以戊二醛固定的对应物作为对照。结果表明，京尼平固定的细胞和脱细胞组织的炎症反应程度明显低于戊二醛固定的组织。另外。人们注意到，戊二醛固定的细胞和非细胞组织的炎症反应比京尼平固定的组织持续的时间要长得多。脱细胞组织再生速度明显快于戊二醛固定的脱细胞组织。各研究组的钙含量经原子吸收分析后，直到术后52周才有明显变化。在整个研究过程中，无论是戊二醛固定组还是京尼平固定组，细胞组织和无细胞组织之间的钙含量差异都不显著。综上所述，京尼平固定的细胞和脱细胞组织的生物相容性优于戊二醛固定的组织。与戊二醛固定的无细胞组织相比，京尼平固定的无细胞组织具有更低的细胞毒性，为组织再生提供了更好的微环境。这些结果提示京尼平固定的脱细胞组织有可能作为组织工程基质应用于临床。(C)2002年由爱思唯尔科学有限公司出版。
A cell extraction process was employed in the study to remove the cellular components from bovine pericardium leaving a framework of largely insoluble collagen and elastin. It was hypothesized in the literature that this process may decrease the antigenic load (or increase the biocompatibility) within the material. Additionally, acellular tissues may provide a natural microenvironment for host-cell migration to regenerate the tissue. The study was to evaluate the biocompatibility of cellular and acellular bovine pericardia fixed with a naturally occurring crosslinking agent (genipin) implanted subcutaneously in a growing rat model. Additionally, the tissue regeneration rate ill the genipin-fixed acellular tissue was investigated. The glutaraldehyde-fixed counterparts were used as controls. The results indicated that the degrees in inflammatory reaction for the genipin-fixed cellular and acellular tissues were significantly less than their glutaraldehyde-fixed counterparts. Additionally. it was noted that the inflammatory reactions for the glutaraldehyde-fixed cellular and acellular tissues lasted much longer than their genipin-fixed counterparts. The tissue regeneration rate for the genipin-fixed acellular tissue was significantly faster than its glutaraldehyde-fixed counterpart. The calcium content of each Studied group, analyzed by atomic absorption, did not change significantly until at the 52nd week, postoperatively. The differences in calcium content between the cellular and acellular tissues were insignificant for both the glutaraldehyde- and genipin-fixed groups throughout the entire course of the study. In summary, the biocompatibility of the genipin-fixed cellular and acellular tissues was superior to their glutaraldehyde-fixed counterparts. The genipin-fixed acellular tissue provided a better microenvironment for tissue regeneration than its glutaraldehyde-fixed counterpart, due to its low cytotoxicity. These results suggested that the genipin-fixed acellular tissue might be used as a tissue-engineering matrix in the clinical applications. (C) 2002 Published by Elsevier Science Ltd.