Differential Staining Of Cobalt‐ And Nickel‐Filled Neurones Using Rubeanic Acid
Differential Staining Of Cobalt‐ And Nickel‐Filled Neurones Using Rubeanic Acid
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使用红豆酸对钴和镍填充的神经元进行差异染色
DOI:
10.1111/j.1365-2818.1979.tb00165.x
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发表时间:
1979
影响因子:
2
通讯作者:
R. C. Brace
中科院分区:
文献类型:
--
作者:
D. Quicke;R. C. Brace
A staining procedure is described which differentiates with distinct colours, cobalt and nickel ions introduced into neurones. Densely coloured precipitates are produced with these ions when rubeanic acid, a spot‐test reagent, is added. Formation of the coloured complexes takes place within a few minutes, and may be followed under the dissecting microscope. This technique permits observation of the relative positions, within ganglia, of somata related to two emergent nerves following multiple axonal‐backfilling. It also appears to be of value in tracing neurone branching after the intracellular injection of ions.