The human umbilical vein: A novel scaffold for musculoskeletal soft tissue regeneration
The human umbilical vein: A novel scaffold for musculoskeletal soft tissue regeneration
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DOI：
10.1111/j.1525-1594.2008.00598.x
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发表时间：
2008-09-01
影响因子：
2.4
通讯作者：
Sikavitsas, Vassilios
中科院分区：
文献类型：
--
作者：
Abousleiman, Rita I.;Reyes, Yuliana;Sikavitsas, Vassilios
脐带是一种容易获得的生物组织，通常被丢弃。在这项研究中，我们调查的潜力，利用部分人脐带，特别是脐静脉，作为一个功能性组织工程支架。以前的研究建议使用人脐静脉（HUV）作为脱细胞血管移植材料。我们建议利用HUV的纵向力学性能，将其用作肌肉骨骼软组织再生的支架材料。从新鲜人脐带的8.5 cm切片上机械解剖HUV。将切片倒置，使管腔侧形成外表面。然后将HUV脱细胞化，并用悬浮在I型胶原水凝胶中的间充质干细胞（MSC）填充。接种的HUV培养长达2周的时间。培养2周后，结果显示细胞数量显著增加，几乎达到原始接种密度的三倍。组织学分析显示，细胞整合和迁移到HUV支架和广泛的细胞外基质重塑。在机械上，极限拉伸应力加倍，弹性模量值几乎高出2.7倍。考虑到MSC的分化能力，沿着适当的生物化学和生物力学环境，接种的HUV具有韧带或肌腱再生的潜力。
The umbilical cord is a biological tissue that is readily available and is usually discarded. In this study, we investigate the potential of making use of part of the human umbilical cord, in particular the umbilical vein, as a functional tissue engineering scaffold. Previous studies suggested the use of the human umbilical vein (HUV) as an acellular vascular grafting material. We propose taking advantage of the longitudinal mechanical properties of the HUV to use it as a scaffold material for musculoskeletal soft tissue regeneration. HUVs were mechanically dissected from 8.5-cm sections of fresh human umbilical cords. The sections were inverted such that the luminal side formed the exterior surface. HUVs were then decellularized, and filled with mesenchymal stem cells (MSCs) suspended in a type I collagen hydrogel. Seeded HUVs were cultured for periods of up to 2 weeks. After 2 weeks of culture, results showed a significant increase in cell number reaching almost three times the original inoculation density. Histological analysis revealed cell integration and migration into the HUV scaffold and extensive remodeling of extracellular matrix. Mechanically, the ultimate tensile stress doubled, and elastic modulus values were almost 2.7-fold higher. Given the differentiation capacity of the MSCs, along with the appropriate biochemical and biomechanical environment, the seeded HUV has a potential for ligament or tendon regeneration.