Chitosan-based nitric oxide-releasing dressing for anti-biofilm and in vivo healing activities in MRSA biofilm-infected wounds
Chitosan-based nitric oxide-releasing dressing for anti-biofilm and in vivo healing activities in MRSA biofilm-infected wounds
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DOI：
10.1016/j.ijbiomac.2019.10.009
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发表时间：
2020-01-01
影响因子：
8.2
通讯作者：
Yoo, Jin-Wook
中科院分区：
文献类型：
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作者：
Choi, Moonjeong;Hasan, Nurhasni;Yoo, Jin-Wook
伤口上的细菌生物膜损害愈合过程，并且常常导致慢性伤口。壳聚糖是一种众所周知的具有抗菌和抗生物膜作用的生物聚合物。S-亚硝基谷胱甘肽（GSNO）是一种很有前途的一氧化氮（NO）供体，可防御病原性生物膜，促进伤口愈合。在这项研究中，我们制备了释放NO的壳聚糖膜（CS/NO膜），并评估其抗生物膜活性和对耐甲氧西林金黄色葡萄球菌（MRSA）生物膜感染的糖尿病小鼠伤口的体内愈合效果。体外释放实验表明，在模拟创面液中，NO的释放可持续3天以上。CS/NO膜通过细菌活力的> 3个对数减少而显著增强针对MRSA的抗菌活性。此外，CS/NO膜表现出比对照和CS膜高3倍的抗生物膜活性。在体内MRSA生物膜感染的伤口中，CS/NO膜处理组显示出比未处理组和CS膜处理组更快的生物膜分散、伤口尺寸减小、上皮形成速率和胶原沉积。因此，本研究中研究的CS/NO膜可能是治疗MRSA生物膜感染伤口的有前途的方法。(C)2019 Elsevier B. V.版权所有。
Bacterial biofilms on wounds impair the healing process and often lead to chronic wounds. Chitosan is a well-known biopolymer with antimicrobial and anti-biofilm effects. S-nitrosoglutathione (GSNO) has been identified as a promising nitric oxide (NO) donor to defend against pathogenic biofilms and enhance wound healing activities. In this study, we prepared NO-releasing chitosan film (CS/NO film) and evaluated its anti-biofilm activity and in vivo wound healing efficacy against methicillin-resistant Staphylococcus aureus (MRSA) biofilm infected wounds in diabetic mice. The in vitro release study showed sustained release of NO over 3 days in simulated wound fluid. The CS/NO film significantly enhanced antibacterial activity against MRSA by > 3 logs reduction in bacterial viability. Moreover, CS/NO film exhibited a 3-fold higher anti-biofilm activity than the control and CS film. In in vivo MRSA biofilm-infected wounds, the CS/NO film-treated group showed faster biofilm dispersal, wound size reduction, epithelialization rates, and collagen deposition than the untreated and CS film-treated groups. Therefore, the CS/NO film investigated in this study could be a promising approach for the treatment of MRSA biofilm-infected wounds. (C) 2019 Elsevier B.V. All rights reserved.