Comparison of mRNA expression from Y-chromosome X-degenerate region genes in taurine cattle, yaks and interspecific hybrid bulls
Comparison of mRNA expression from Y-chromosome X-degenerate region genes in taurine cattle, yaks and interspecific hybrid bulls
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牛磺酸牛、牦牛和种间杂交公牛Y染色体X简并区基因mRNA表达比较
DOI：
10.1111/age.12841
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发表时间：
2019
期刊：
影响因子：
2.4
通讯作者：
Zhang G. -W.
中科院分区：
文献类型：
--
作者：
Wu Y.;Zhang W. -X.;Zuo F.;Zhang G. -W.
牦牛（DZO）是牛磺酸牛（Bos taurus）和家牦牛（Bos grunniens）的种间杂种。F1代杂种雅特公牛不育是由于精子发生停滞和睾丸中精子发生基因的失调。然而，Y染色体连锁基因在牛、牦牛和雅特牛睾丸中的表达模式仍然未知。在这项研究中，我们分析了10个基因的mRNA表达模式，这些基因已知以单拷贝形式存在于Y染色体牛雄性特异性区域的X简并区域。利用雄性特异性引物和逆转录定量PCR，泛转录的三肽重复基因Y连锁（UTY），口面指综合征1，Y连锁（OFD 1 Y），抗胰蛋白酶特异性肽酶9，Y连锁（USP 9 Y）基因在牦牛睾丸中普遍表达，并且显著高于牛和牦牛睾丸RNA结合基序蛋白Y连锁（RBMY）在睾丸中有特异性表达，真核翻译起始因子1A Y连锁（EIF1AY）主要在睾丸中表达，而牦牛和牛在RBMY和EIF1AY的表达方面没有表现出显著差异。因此，基于公鸭不育模型，UTY、OFD 1Y和USP 9Y的高表达可能与公鸭不育有关。
The yattle (dzo) is an interspecific hybrid of the taurine cattle (Bos taurus) and the domestic yak (Bos grunniens). F1hybrid yattle bulls are sterile due to spermatogenic arrest and have misregulation of spermatogenesis genes in the testes. However, the expression pattern of Y chromosome‐linked genes in cattle, yaks and yattle testes is still unknown. In this study, we analyzed the mRNA expression pattern of 10 genes known to be present as single copies in the X‐degenerate region of the bovine male‐specific region of the Y chromosome. Using male‐specific primers and reverse transcription quantitative PCR, theubiquitously transcribed tetratricopeptide repeat gene, Y‐linked(UTY),oral‐facial‐digital syndrome 1, Y‐linked(OFD1Y) andubiquitin specific peptidase 9, Y‐linked(USP9Y) genes were ubiquitously expressed and significantly more highly expressed in yattle than in cattle and yaks testes (P<0.001).RNA binding motif protein, Y‐linked(RBMY) had testes‐specific expression, andeukaryotic translation initiation factor 1A, Y‐linked(EIF1AY) was expressed mainly in testis, whereas yattle and cattle did not show significant differences with respect to the expression ofRBMYandEIF1AY. Thus, based on the model of yattle bull sterility, the high expression ofUTY,OFD1YandUSP9Ymay be associated with yattle infertility.