STRUCTURES OF ENZYMATICALLY MODIFIED PRODUCTS OF ARBEKACIN BY METHICILLIN-RESISTANT
STRUCTURES OF ENZYMATICALLY MODIFIED PRODUCTS OF ARBEKACIN BY METHICILLIN-RESISTANT
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DOI：
10.7164/antibiotics.46.310
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发表时间：
1993-02-01
影响因子：
3.3
通讯作者：
MITSUHASHI, S
中科院分区：
文献类型：
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作者：
KONDO, S;TAMURA, A;MITSUHASHI, S
临床上仅分离出有限数量的耐甲氧西林金黄色葡萄球菌（MRSA），对阿贝卡星（ABK）中度耐药。通过与过量的从ABK-抗性MRSA菌株（MIC，25 μ g/ml）提取的粗酶制剂反应，获得了ABK的三种灭活产物。2“-O-磷酸酯是主要产物，还有少量的6”-N-乙酸酯和双重修饰产物。通过质谱和核磁共振谱分析确定了改性产物的结构。
Only a limited number of strains of methicillin-resistant Staphylococcus aureus (MRSA) moderately resistant to arbekacin (ABK) have been isolated clinically. Three inactivated products of ABK have been obtained by reaction with excess amounts of a crude enzyme preparation extracted from an ABK-resistant MRSA strain (MIC, 25 mug/ml). The 2''-O-phosphate was the major product together with small amounts of the 6'-N-acetate and the double modification product. The structures of these modification products were determined by MS and NMR spectral analyses.