Modulation of vacuolar H+ -pumps and aquaporin by phytohormones in rice seedling leaf sheaths.
Modulation of vacuolar H+ -pumps and aquaporin by phytohormones in rice seedling leaf sheaths.
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DOI：
10.1248/bpb.26.88
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发表时间：
2003
影响因子：
2
通讯作者：
Shihai Yang;M. Maeshima;Yoshiyuki Tanaka;S. Komatsu
中科院分区：
文献类型：
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作者：
Shihai Yang;M. Maeshima;Yoshiyuki Tanaka;S. Komatsu
研究了外源赤霉酸(GA(3))或油菜素内酯(BL)对水稻幼苗叶鞘液泡膜中H(+)-泵和水通道蛋白的影响。绿豆H(+)-PPase、V-ATPase B亚基和萝卜液泡膜内在蛋白(TIP)的抗体与水稻幼苗叶鞘的液泡膜蛋白发生交叉反应。免疫印迹分析结果显示，在GA(3)存在下，H(+)-PPase和V-ATPase含量在两天内保持在较高水平，而在没有植物激素的情况下，H(+)-PPase和V-ATPase含量逐渐下降，表明GA(3)对液泡H(+)-泵的活性有促进作用。然而，用 BL 处理后，V-ATPase 和 H(+)-PPase 水平在第 2 天升高，然后降低。 GA(3) 和 BL 治疗后 TIP 水平没有明显差异。与水对照相比，BL处理后的H(+)-PPase活性也保持在较高水平，说明BL对H(+)-PPase活性有促进作用。这些结果表明GA(3)和BL可能参与植物液泡H(+)-泵的数量和活性的调节。
The effects of exogenously applied gibberellic acid (GA(3)) or brassinolide (BL) on the H(+)-pumps and aquaporin in the vacuolar membrane of rice seedling leaf sheath were investigated. Antibodies against mung bean H(+)-PPase, the B subunit of V-ATPase, and radish tonoplast intrinsic protein (TIP) cross-reacted with the vacuolar membrane proteins of rice seedling leaf sheath. The results of immunoblot analysis showed that the amounts of H(+)-PPase and V-ATPase were retained at a high level for two days in the presence of GA(3), although the level gradually decreased without phytohormones, indicating that GA(3) has a promotive effect on the activities of vacuolar H(+)-pumps. However, the levels of V-ATPase and H(+)-PPase were increased on day 2 and then decreased when treated with BL. There were no visible differences in the level of TIP upon treatment with GA(3) and BL. In comparison with the water control, the activity of H(+)-PPase treated with BL was also retained at a relatively high level, suggesting that BL has a stimulative effect on the activities of H(+)-PPase. These results indicate that GA(3) and BL might be involved in the regulation of the quantity and activities of plant vacuolar H(+)-pumps.