Quantitative Trait Loci Controlling Phytophthora cactorum Resistance in the Cultivated Octoploid Strawberry ( Fragaria x ananassa )
Quantitative Trait Loci Controlling Phytophthora cactorum Resistance in the Cultivated Octoploid Strawberry ( Fragaria x ananassa )
复制标题
控制栽培八倍体草莓（草莓）疫霉抗性的数量性状位点
DOI：
10.1101/249573
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nellist C
中科院分区：
文献类型：
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作者：
Nellist C
栽培草莓（Fragaria× ananassa（Fragaria spp.）是全球最具经济价值的软果。恶疫霉是一种水传卵菌，在全球草莓生产中造成经济损失。采用人工接种的方法，对四倍体栽培草莓进行了抗蔓枯病的双亲分离鉴定。多个推定的抗性数量性状位点（QTL）进行了鉴定和定位。三个主效QTL（FaRPc6C、FaRPc6D和FaRPc7D）解释了37%的变异。3个主效QTL之间均未检测到上位性互作。对一系列恶疫霉分离株的作图群体后代的子集进行测试，没有发现显著的相互作用（p= 0.0593）。然而，一些品系表现出比预测的更高的感病性，表明额外的未检测到的因素可能会影响一些数量抗性位点的表达。利用草莓品种的冠腐病评分数据，对114个个体进行了初步的全基因组关联研究（GWAS），发现了一个与对P. cactorum抗性相关的额外位点。草莓的开采夏威夷4 v1. 1基因组中的QTL区域揭示了候选抗性基因。
The cultivated strawberry, Fragaria× ananassa (Fragaria spp.) is the most economically important global soft fruit. Phytophthora cactorum, a water-borne oomycete causes economic losses in strawberry production globally. A bi-parental cross of octoploid cultivated strawberry segregating for resistance to P. cactorum, the causative agent of crown rot disease, was screened using artificial inoculation. Multiple putative resistance quantitative trait loci (QTL) were identified and mapped. Three major effect QTL (FaRPc6C, FaRPc6D and FaRPc7D) explained 37% of the variation observed. There were no epistatic interactions detected between the three major QTLs. Testing a subset of the mapping population progeny against a range of P. cactorum isolates revealed no significant interaction (p= 0.0593). However, some lines showed higher susceptibility than predicted, indicating that additional undetected factors may affect the expression of some quantitative resistance loci. Using historic crown rot disease score data from strawberry accessions, a preliminary genome-wide association study (GWAS) of 114 individuals revealed an additional locus associated with resistance to P. cactorum. Mining of the Fragaria vesca Hawaii 4 v1. 1 genome revealed candidate resistance genes in the QTL regions.