Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup.
Comparative studies on the structure of the light chains of human immunoglobulins. IV. Assignment of a subsubgroup.
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人免疫球蛋白轻链结构的比较研究。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
A. Shimizu
中科院分区：
文献类型：
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作者：
F. Kametani;T. Takayasu;S. Suzuki;T. Shinoda;T. Okuyama;A. Shimizu
对人 lambda Bence Jones 蛋白 NIG-64 进行氨基酸序列分析，主要目的是确定可变区的序列。从完全还原和氨乙基化的蛋白质中分离出19个涵盖216个残基的胰蛋白酶肽，其中17个已完成测序。这些包括整个可变区和 11 个恒定区。对于覆盖其余恒定区的剩余肽，仅确定了部分序列或氨基酸组成。根据上述结果以及与相同同种型的其他人λ轻链的同源性，所有胰蛋白酶肽可以按顺序排列。与同一亚群的其他蛋白相比，该蛋白的可变区序列与V lambda I亚群的New蛋白高度同源，提示V lambda I亚群可能进一步分为亚亚群，即亚亚群V lambda I-1和V lambda I-2。
Amino acid sequence analysis was done on a human lambda Bence Jones protein NIG-64 with the major objective of determining the sequence of the variable region. Nineteen tryptic peptides covering 216 residues were isolated from the completely reduced and aminoethylated protein, and 17 of these were completely sequenced. These comprised the entire variable region and 11 from the constant region. For the remaining peptides covering the rest of the constant region, only partial sequences or the amino acid compositions were determined. All the tryptic peptides could be arranged in order on the basis of the above results and homology with other human lambda light chains of the same isotype. The sequence of the variable region of the protein is highly homologous with that of protein New of subgroup V lambda I as compared with other proteins of the same subgroup, suggesting that subgroup V lambda I may be further divided into subsubgroups, namely subsubgroups V lambda I-1 and V lambda I-2.