Quantitative proteomic analysis of Rhodococcus ruber responsive to organic solvents
Quantitative proteomic analysis of Rhodococcus ruber responsive to organic solvents
复制标题
DOI：
10.1080/13102818.2018.1533432
复制
发表时间：
2018-11
影响因子：
1.4
通讯作者：
Sufang Kuang;Xin Fan;R. Peng
中科院分区：
文献类型：
--
作者：
Sufang Kuang;Xin Fan;R. Peng
摘要红色红球菌具有良好的有机耐受性，在生物转化和生物修复方面具有潜在的应用价值。为探讨可能的机体耐受机制，研究了R.采用定量蛋白质组学方法（iTRAQ）和液相色谱-串联质谱（LC-MS）研究了ruberSD 3对甲苯和苯酚的降解作用。与对照组相比，在甲苯处理组和苯酚处理组中分别鉴定出总共362和488个差异表达蛋白。功能注释和代谢途径富集表明，转运蛋白、降解途径和双组分系统与红曲霉的有机溶剂耐受性密切相关。红宝石SD 3实时定量聚合酶链反应实验表明，与对照相比，甲苯胁迫后应激蛋白的mRNA水平增加了3.23倍。苯酚处理组4-硝基苯酚2-单加氧酶的表达是对照组的123倍。本研究揭示了可能的耐药机制。ruberSD 3对有机溶剂胁迫的耐受性，为R.橡胶SD 3以提高其有机溶剂耐受性。
Abstract Rhodococcus ruber with organic tolerance has potential applications in biotransformation and bioremediation. To explore the possible organic tolerance mechanism, the response of R. ruber SD3 to toluene and phenol was investigated using a quantitative proteomics approach with isobaric tag for relative and absolute quantification (iTRAQ) and liquid chromatography-tandem mass spectrometry. A total of 362 and 488 differentially expressed proteins were identified in the toluene treatment group and the phenol treatment group as compared to the control group, respectively. Functional annotation and metabolic pathway enrichment showed that transporter, degradation pathway and two-component system were closely related to organic solvent tolerance of R. ruber SD3. The quantitative real-time polymerase chain reaction experiment indicated the mRNA levels of stress proteins with an increased expression of 3.23 times upon toluene stress as compared to the control. The expression of 4-nitrophenol 2-monooxygenase in the phenol treatment group was ∼123 times higher than the counterpart in the control group. The study revealed the possible tolerance mechanism of R. ruber SD3 to organic solvents stress and provided some potential targets for the engineering of R. ruber SD3 to improve its organic solvent tolerance.