Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries
Myoblast proliferation and differentiation on fibronectin-coated self assembled monolayers presenting different surface chemistries
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DOI：
10.1016/j.biomaterials.2004.11.028
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发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
García, AJ
中科院分区：
文献类型：
--
作者：
Lan, MA;Gersbach, CA;García, AJ
生物材料的表面性质调节蛋白质吸附和细胞粘附，以引起生物医学和生物技术应用中的各种细胞反应。我们使用链烷醇自组装单层呈现明确的化学成分（OH，CH 3，NH 2和COOH）来分析表面化学对成肌细胞增殖和分化的影响。表面用等密度的纤连蛋白预包被。C2 C12骨骼肌成肌细胞表现出细胞增殖的表面依赖性差异（COOH = NH 2> CH 3 = OH）。与其他化学物质相比，肌生成素和肌钙蛋白T基因表达水平在CH 3和OH表面上上调。此外，肌节肌球蛋白的免疫染色揭示了肌源性分化的表面化学依赖性差异，其模式为OH > CH 3> NH 2 = COOH。整合素亚基的免疫染色分析表明，表面化学依赖的差异，整合素结合吸附的纤连蛋白。OH和CH 3表面支持α（5）β（1）整联蛋白的选择性结合，而COOH和NH 2官能团显示α（5）β（1）和α（v）β（3）两者的结合。肌源性分化与整合素结合的差异相关;支持α（5）β（1）选择性结合的表面化学表现出增强的分化。最后，阻断β（1），而不是β（3），整合素抑制分化，暗示分化过程中的特定整合素。这些结果表明，表面化学调节成肌细胞的增殖和分化，通过整合素结合吸附的纤连蛋白的差异。(c)2004爱思唯尔有限公司保留所有权利。
Biomaterial surface properties modulate protein adsorption and cell adhesion to elicit diverse cellular responses in biomedical and biotechnological applications. We used alkanethiol self-assembled monolayers presenting well-defined chemistries (OH, CH3, NH2, and COOH) to analyze the effects of surface chemistry on myoblast proliferation and differentiation. Surfaces were pre-coated with equivalent densities of fibronectin. C2C12 skeletal myoblasts exhibited surface-dependent differences in cell proliferation (COOH = NH2 > CH3 = OH). Myogenin and troponin T gene expression levels were up-regulated on CH3 and OH surfaces compared to other chemistries. Furthermore, immunostaining for sarcomeric myosin revealed surface chemistry-dependent differences in myogenic differentiation following the pattern OH > CH3 > NH2 = COOH. Immunostaining analyses of integrin subunits demonstrated surface chemistry-dependent differences in integrin binding to adsorbed fibronectin. OH and CH3 surfaces supported selective binding Of alpha(5)beta(1) integrin while the COOH and NH2, functionalities displayed binding of both alpha(5)beta(1), and alpha(v)beta(3). Myogenic differentiation correlated with differences in integrin binding; surface chemistries that supported selective binding Of alpha(5)beta(1) displayed enhanced differentiation. Finally, blocking beta(1), but not beta(3), integrins inhibited differentiation, implicating specific integrins in the differentiation process. These results demonstrate that Surface chemistry modulates myoblast proliferation and differentiation via differences in integrin binding to adsorbed fibronectin. (c) 2004 Elsevier Ltd. All rights reserved.