Turns in transmembrane helices:: Determination of the minimal length of a "helical hairpin" and derivation of a fine-grained turn propensity scale
Turns in transmembrane helices:: Determination of the minimal length of a "helical hairpin" and derivation of a fine-grained turn propensity scale
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DOI：
10.1006/jmbi.1999.3183
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发表时间：
1999-11-05
影响因子：
5.6
通讯作者：
von Heijne, G
中科院分区：
文献类型：
--
作者：
Monné, M;Nilsson, I;von Heijne, G
我们最近报道了第一个实验转跨膜螺旋倾向规模。该标度来源于对放置在40个残基聚（Leu）延伸中间的给定残基如何有效地诱导具有两个而不是一个跨膜区段的“螺旋发夹”的形成的测量。我们现在已经扩展了这些研究，并确定了与螺旋发夹形成相容的聚（亮氨酸）延伸的最小长度。我们还通过（i）将20个氨基酸残基中的每一个引入与螺旋发夹形成相容的最短聚（Leu）延伸的中间，以及（ii）在40个残基聚（Leu）延伸的中间引入残基对，推导出更细粒度的转弯倾向量表。新的转向倾向与已知3D结构的膜蛋白中短发夹环中发现的氨基酸频率一致。(C)北京：科学出版社.
We have recently reported a first experimental turn propensity scale for transmembrane helices. This scale was derived from measurements of how efficiently a given residue placed in the middle of a 40 residue poly(Leu) stretch induces the formation of a "helical hairpin" with two rather than one transmembrane segment. We have now extended these studies, and have determined the minimum length of a poly(Leu) stretch compatible with the formation of a helical hairpin. We have also derived a more fine-grained turn propensity scale by (i) introducing each of the 20 amino acid residues into the middle of the shortest poly(Leu) stretch compatible with helical hairpin formation, and (ii) introducing pairs of residues in the middle of the 40 residue poly(Leu) stretch. The new turn propensities are consistent with the amino acid frequencies found in short hairpin loops in membrane proteins of known 3D structure. (C) 1999 Academic Press.