The effects of aging and oxidative stress on learning behavior in C. elegans
The effects of aging and oxidative stress on learning behavior in C. elegans
复制标题
DOI：
10.1016/j.neurobiolaging.2004.08.007
复制
发表时间：
2005-06-01
影响因子：
4.2
通讯作者：
Murakami, H
中科院分区：
文献类型：
--
作者：
Murakami, S;Murakami, H
氧化应激与年龄相关的生物功能下降有关。然而，秀丽隐杆线虫的神经系统在衰老过程中得以保存，因此，还没有很好地探讨衰老和氧化应激是否会影响神经功能。在这里，我们报告说，年龄相关的下降可以观察到一种类型的联想学习行为，称为等温跟踪。我们还报道了对氧化应激敏感性改变的突变体对年轻人学习行为和运动活动的影响。isp-1和isp-1突变体是Clk类突变体的成员，并且在线粒体呼吸链的功能中具有缺陷，导致氧化应激水平降低、寿命延长、节律行为延迟和其他表型。Clk突变和用代谢性抗氧化剂α-硫辛酸（LA）预处理都增加了显示等温跟踪的能力，并适度降低了运动活性。氧化应激增加的突变体表现出学习行为严重受损和运动活动适度减少。因此，氧化应激的生理水平可能对学习行为来说太高，但对运动活动来说可能不是。我们讨论了氧化应激与衰老和行为演变的相关性。(c)2004年爱思唯尔公司All rights reserved.
Oxidative stress is associated with age-related declines of biological functions. However, the nervous system is preserved during aging in Caenorhabditis elegans and, thus, it is not well explored whether aging and oxidative stress affect nervous functions. Here we report that age-related decline can be observed in a type of associative-learning behavior, referred to as isothermal tracking. We also report the effects of mutants with altered sensitivity to oxidative stress on learning behavior and motor activity in young adults. The isp-1 and clk-1 mutants are members of the Clk class of mutants and have deficits in the function of the mitochondrial respiratory chain, leading to reduced levels of oxidative stress, increased longevity, delayed rhythmic behaviors and other phenotypes. Both the Clk mutations and pretreatment with a metabolic antioxidant, alpha-lipoic acid (LA), increased the ability to show isothermal tracking and modestly reduced motor activity. Mutants with increased oxidative stress showed severely impaired learning behavior and modestly reduced motor activity. Therefore, physiological levels of oxidative stress may be too high for learning behavior but, perhaps, not for motor activity. We discuss the relevance of oxidative stress to the aging and evolution of behaviors. (c) 2004 Elsevier Inc. All rights reserved.